Genetic analysis of BRCA1 function in a defined tumor cell line

被引：309

作者：

Scully, R

Ganesan, S

Vlasakova, K

Chen, JJ

Socolovsky, M

Livingston, DM ^{[1
]}

机构：

[1] Dana Farber Canc Inst, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Boston, MA 02115 USA

[3] Whitehead Inst, Cambridge, MA 02138 USA

[4] MIT, Cambridge, MA 02138 USA

来源：

MOLECULAR CELL | 1999年 / 4卷 / 06期

关键词：

D O I：

10.1016/S1097-2765(00)80238-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Retrovirally expressed, wild-type BRCA1 decreased the gamma radiation (IR) sensitivity and increased the efficiency of double-strand DNA break repair (DSBR) of the BRCA1(-/-) human breast cancer line, HCC1937. It also reduced its susceptibility to DSB generation by IR. By contrast, multiple, clinically validated, missense mutant BRCA1 products were nonfunctional in these assays. These data constitute the basis for a BRCA1 functional assay and suggest that efficient repair of double-strand DNA breaks is linked to BRCA1 tumor suppression function.

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页码：1093 / 1099

页数：7

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