Inhibition of Hepatitis B virus replication by SAMHD1

被引：80

作者：

Chen, Zhangming ^{[1
]}

Zhu, Mengying ^{[1
]}

Pan, Xiang ^{[1
]}

Zhu, Yongji ^{[1
]}

Yan, Hai ^{[1
]}

Jiang, Tongcui ^{[2
,3
]}

Shen, Yujun ^{[2
,3
]}

Dong, Xiaowan ^{[1
]}

Zheng, Nan ^{[1
]}

Lu, Jinsen ^{[1
]}

Ying, Songcheng ^{[1
]}

Shen, Yuxian ^{[2
,3
]}

机构：

[1] Anhui Med Univ, Sch Basic Med Sci, Dept Immunol, Hefei 230032, Anhui, Peoples R China

[2] Anhui Med Univ, Sch Basic Med Sci, Hefei 230032, Anhui, Peoples R China

[3] Anhui Med Univ, Biopharmaceut Res Inst, Hefei 230032, Anhui, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 450卷 / 04期

基金：

中国国家自然科学基金;

关键词：

SAMHD1; HBV; IFN-alpha; Liver cell; AICARDI-GOUTIERES SYNDROME; RESTRICTION FACTOR SAMHD1; X-PROTEIN; IN-VITRO; HIV-1; ENHANCEMENT; MACROPHAGES; INFECTION; IMMUNITY; TYPE-1;

D O I：

10.1016/j.bbrc.2014.07.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Sterile alpha motif and HD-domain containing protein 1 (SAMHD1) is a newly identified intracellular antiviral factor. By depleting the dNTPs pool of host cells to a low level that cannot support the efficient synthesis of viral cDNA, it restricts replication of some retroviruses. As a DNA virus, Hepatitis B virus (HBV) experiences a process of reverse transcription in its life cycle akin to that of retroviruses. However, whether SAMHD1 can restrict HBV replication in liver cells is unknown. Here, we reported that SAMHD1 expression was detectable in four liver cell lines. Exogenous expression of SAMHD1 in SMMC-7721 cells restrained HBV replication. Similarly, SAMHD1 impeded HBV replication in another liver cell line, BEL-7402. Remarkably, the catalytically inactive mutant, SAMHD1 HD/AA also hampered HBV replication. Additionally, HBV replication reduced SAMHD1 expression in HepG2 cells. Moreover, it was found that IFN-alpha induced expression of SAMHD1 in liver cells. Together, these findings suggested that IFN-alpha-inducible SAMHD1 inhibited HBV replication in liver cells. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：1462 / 1468

页数：7

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