Chromatin studies by DNA-protein cross-linking

被引：10

作者：

Pruss, D

Bavykin, SG

机构：

[1] NICHHD, MOL EMBRYOL LAB, NIH, BETHESDA, MD 20892 USA

[2] RUSSIAN ACAD SCI, VA ENGELHARDT MOL BIOL INST, MOSCOW 117984, RUSSIA

来源：

METHODS | 1997年 / 12卷 / 01期

关键词：

D O I：

10.1006/meth.1997.0445

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Our current level of understanding of chromatin structure was to a large extent achieved with the help of DNA-protein cross-linking. The versatile inventory of cross-linking techniques allows the identification of the contacts between DNA and proteins with a single nucleotide-single amino acid precision, to detect minor components of the complex nucleoprotein systems, to reveal the interactions of the flexible protein domains with DNA, and to assay for conformational changes in the nucleosomes. (C) 1997 Academic Press.

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页码：36 / 47

页数：12

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