HISTONE H1 DEPOSITION AND HISTONE DNA INTERACTIONS IN REPLICATING CHROMATIN

被引：29

作者：

BAVYKIN, S

SREBREVA, L

BANCHEV, T

TSANEV, R

ZLATANOVA, J

MIRZABEKOV, A

机构：

[1] BULGARIAN ACAD SCI, INST MOLEC BIOL, BU-1113 SOFIA, BULGARIA

[2] BULGARIAN ACAD SCI, INST GENET, BU-1113 SOFIA, BULGARIA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 09期

关键词：

CHROMATIN REPLICATION; HISTONE DNA CROSS-LINKING; IMMUNOCHEMISTRY;

D O I：

10.1073/pnas.90.9.3918

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An immunochemical method for analyzing protein interactions with BrdUrd-substituted DNA was used to study binding of histones to nascent DNA in nuclei. The results indicate that in Ehrlich ascites tumor (EAT) cells, histone H1 deposits on newly replicated DNA simultaneously with or immediately after core histone deposition so that in chromatin replicated for 3 min, the stoichiometry of the histones is the same as in bulk chromatin. All histones, and especially histone H1, interact with nascent DNA more weakly than with bulk chromatin, although the efficiency of interaction via the globular domains seems to be the same for both types of chromatin.

引用

页码：3918 / 3922

页数：5

共 45 条

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