Prospective hatching of embryos developed from oocytes exhibiting difficult oolemma penetration during ICSI

被引:22
作者
Ebner, T [1 ]
Moser, M [1 ]
Yaman, C [1 ]
Sommergruber, M [1 ]
Hartl, J [1 ]
Jesacher, K [1 ]
Tews, G [1 ]
机构
[1] Womens Gen Hosp Linz, IVF Unit, A-4020 Linz, Upper Austria, Austria
关键词
assisted reproductive techniques; ICSI; oolema penetration; ovarian stimulation;
D O I
10.1093/humrep/17.5.1317
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
BACKGROUND: The hormonal milieu during ovarian stimulation is known to affect oolemma behaviour as well as zona pellucida thickness and structure. This led us to investigate whether a special subgroup of patients with oocytes where penetration of the oolemma is difficult during ICSI may benefit from assisted hatching. METHODS: A total of 77 couples (mean age: 32.9+/-4.6 years; range: 22-38) had oocytes that could hardly be penetrated by the ICSI pipette. Nineteen patients underwent two ICSI cycles, giving a total number of 96 cycles, which were randomly split into either the study group (n=52) or the non-hatching group (n=44). Hatching was done using a non-contact 1.48 mm wavelength diode laser. Implantation and pregnancy rates were recorded. RESULTS: The pregnancy rate was 36.6% (19/52) in the study group and 13.6% (6/44) in the non-hatching group (P<0.05). In addition, a higher number (P<0.05) of embryos implanted in the study group (23/106; 21.7%) than in the non-hatching group (9/92; 9.8%). CONCLUSIONS: Once oolema penetration during ICSI has proven difficult, prospective hatching of embryos considered for transfer may increase their implantation behaviour.
引用
收藏
页码:1317 / 1320
页数:4
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