A three-component enzyme system catalyzes the O demethylation of the herbicide dicamba in Pseudomonas maltophilia DI-6

被引：33

作者：

Wang, XZ ^{[1
]}

Li, B ^{[1
]}

Herman, PL ^{[1
]}

Weeks, DP ^{[1
]}

机构：

[1] UNIV NEBRASKA, CTR BIOTECHNOL, GEORGE W BEADLE CTR N300, DEPT BIOCHEM, LINCOLN, NE 68588 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1997年 / 63卷 / 04期

关键词：

D O I：

10.1128/AEM.63.4.1623-1626.1997

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An enzyme activity which converts dicamba (2-methoxy-3,6-dichlorobenzoic acid) to 3,6-dichlorosalicyclic acid in vitro has been detected in cell lysates of Pseudomnoas maltophilia DI-6. Phenyl-Sepharose column chromatography of a partially purified lysate resulted in the separation of this enzyme into three separate protein components tentatively identified as an oxygenase, a ferredoxin, and a reductase. The activity of dicamba O-demethylase was dependent on oxygen and required NADH and Mg2+.

引用

页码：1623 / 1626

页数：4

共 21 条

[1]

[Anonymous], 1991, Chemistry and Biochemistry of Flavoenzymes

[2] PURIFICATION AND SOME PROPERTIES OF A SOLUBLE BENZENE-OXIDIZING SYSTEM FROM A STRAIN OF PSEUDOMONAS [J].

AXCELL, BC ;

GEARY, PJ .

BIOCHEMICAL JOURNAL, 1975, 146 (01) :173-183

[3] 4-METHOXYBENZOATE O-DEMETHYLASE FROM PSEUDOMONAS-PUTIDA - NEW TYPE OF MONOOXYGENASE SYSTEM [J].

BERNHARDT, FH ;

PACHOWSKY, H ;

STAUDINGER, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1975, 57 (01) :241-256

[4] CLONING AND SEQUENCING OF PSEUDOMONAS GENES ENCODING VANILLATE DEMETHYLASE [J].

BRUNEL, F ;

DAVISON, J .

JOURNAL OF BACTERIOLOGY, 1988, 170 (10) :4924-4930

[5]

CORK DJ, 1991, ADV APPL MICROBIOL, V36, P1

[6] OXIDATION OF NAPHTHALENE BY A MULTICOMPONENT ENZYME-SYSTEM FROM PSEUDOMONAS SP STRAIN NCIB9816 [J].

ENSLEY, BD ;

GIBSON, DT ;

LABORDE, AL .

JOURNAL OF BACTERIOLOGY, 1982, 149 (03) :948-954

[7] PURIFICATION AND SOME PROPERTIES OF 2-HALOBENZOATE 1,2-DIOXYGENASE, A 2-COMPONENT ENZYME-SYSTEM FROM PSEUDOMONAS-CEPACIA 2CBS [J].

FETZNER, S ;

MULLER, R ;

LINGENS, F .

JOURNAL OF BACTERIOLOGY, 1992, 174 (01) :279-290

[8] OXIDATION OF BIPHENYL BY A MULTICOMPONENT ENZYME-SYSTEM FROM PSEUDOMONAS SP STRAIN LB400 [J].

HADDOCK, JD ;

NADIM, LM ;

GIBSON, DT .

JOURNAL OF BACTERIOLOGY, 1993, 175 (02) :395-400

[9] FUNCTIONAL AND EVOLUTIONARY RELATIONSHIPS AMONG DIVERSE OXYGENASES [J].

HARAYAMA, S ;

KOK, M ;

NEIDLE, EL .

ANNUAL REVIEW OF MICROBIOLOGY, 1992, 46 :565-601

[10] ISOLATION AND IDENTIFICATION OF MICROORGANISMS FOR THE DEGRADATION OF DICAMBA [J].

KRUEGER, JP ;

BUTZ, RG ;

ATALLAH, YH ;

CORK, DJ .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1989, 37 (02) :534-538

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