Synthesis of heptapeptides and analysis of sequence by tandem ion trap mass spectrometry

被引：9

作者：

Jia, Chenxi ^{[1
]}

Qi, Wei ^{[1
]}

He, Zhimin ^{[1
]}

Yang, Haoming ^{[1
]}

Qiao, Bin ^{[1
]}

机构：

[1] Tianjin Univ, Chem Engn Res Ctr, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China

来源：

CENTRAL EUROPEAN JOURNAL OF CHEMISTRY | 2006年 / 4卷 / 02期

基金：

中国国家自然科学基金;

关键词：

peptide synthesis; mass spectrometry; peptide sequencing; racemization; RP-HPLC; scavenger;

D O I：

10.2478/s11532-006-0004-6

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two heptapeptides have been prepared by Fmoc methodology using Wang resin as solid support. For attachment of the first. amino acid, several coupling systems were evaluated, and DIC/DMAP system could give yields of >99 % and low levels of racemization. The selection of scavenger combination to deprotect side chains revealed that H2O/p-cresol was good at scavenging trityl and 1,2-ethanedithiol was highly efficient for scavenging t-butyl. Through shortening the preactivation time to 5 min, the racemization which occurred during formation of amide bonds coupled by HBTU was minimized. The crude peptides were characterized by RP-HPLC and MS, and sequenced by MS/MS to acquire reliable amino acid sequence information. (C) Versita Warsaw and Springer-Verlag Berlin Heidelberg. All rights reserved.

引用

页码：285 / 298

页数：14

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