Size and complexity of the nuclear genome of the ectomycorrhizal fungus Paxillus involutus

被引：19

作者：

Le Quéré, A ^{[1
]}

Johansson, T ^{[1
]}

Tunlid, A ^{[1
]}

机构：

[1] Lund Univ, Dept Microbial Ecol, S-22362 Lund, Sweden

来源：

FUNGAL GENETICS AND BIOLOGY | 2002年 / 36卷 / 03期

关键词：

Paxillus involutus; basidiomycete; ectomycorrhiza; DNA reassociation kinetics; genomic reconstruction; cosmid sequencing; vegetative incompatibility protein;

D O I：

10.1016/S1087-1845(02)00024-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The basidiomycete Paxillus involutus is forming ectomycorrhizal symbiosis with a broad range of forest trees. Reassociation kinetics on P. involutus nuclear DNA indicated a haploid genome size of 23 Mb including 11% of repetitive DNA. A similar genome size (20 Mb) was estimated by genomic reconstruction analysis using three single copy genes. To assess the gene density in the P. involutus genome, a cosmid containing a 33-kb fragment of genomic DNA was sequenced and used to identify putative open reading frames (ORFs). Twelve potential ORFs were predicted, eight displayed significant sequence similarities to known proteins found in other organisms and notably, several homologues to the Podospora anserina vegetative incompatibility protein (HetE1) were found. By extrapolation, we estimate the total number of genes in the P. involutus haploid genome to approximately 7700. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：234 / 241

页数：8