Osteogenic Differentiation Gene Expression Profiling of hMSCs on Hydroxyapatite and Mineralized Collagen

被引：12

作者：

Xu, Su-Ju ^{[1
,2
]}

Qiu, Zhi-Ye ^{[1
]}

Wu, Jing-Jing ^{[1
,3
]}

Kong, Xiang-Dong ^{[2
]}

Weng, Xi-Sheng ^{[4
,5
]}

Cui, Fu-Zhai ^{[1
]}

Wang, Xiu-Mei ^{[1
]}

机构：

[1] Tsinghua Univ, Sch Mat Sci & Engn, State Key Lab New Ceram & Fine Proc, Beijing 100084, Peoples R China

[2] Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou, Zhejiang, Peoples R China

[3] China Univ Geosci, Sch Engn & Technol, Beijing, Peoples R China

[4] Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Orthopaed Surg, Beijing 100730, Peoples R China

[5] Peking Union Med Coll, Beijing 100021, Peoples R China

来源：

TISSUE ENGINEERING PART A | 2016年 / 22卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

MESENCHYMAL STEM-CELLS; IN-VITRO; BONE-FORMATION; BEHAVIOR; OSTEOINDUCTION; BIOMATERIALS; MECHANISM; SCAFFOLD;

D O I：

10.1089/ten.tea.2015.0237

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

In this study, human mesenchymal stem cells (hMSCs) were cultured on the hydroxyapatite (HA) and mineralized collagen (MC), and their proliferation, adhesion, and differentiation, especially the molecular mechanisms on gene level, were investigated. Proliferation and morphological responses of hMSCs and their osteogenic differentiation were detected by quantitative detection of alkaline phosphatase. Gene expression profilings were examined by microarrays, and the gene expression data were studied through gene ontology terms and pathway analyses. The results showed that MC promoted cell proliferation and osteogenic differentiation of hMSCs. Microarray analysis showed that MC was conducive to express osteogenesis-related genes, such as BMP-2, COL1A1, and CTSK, and stimulate osteogenic differentiation, such as osteoblast differentiation pathway and skeletal system development pathway.

引用

页码：170 / 181

页数：12