Interaction of distamycin A and netropsin with quadruplex and duplex structures:: A comparative 1H-NMR study

被引：33

作者：

Randazzo, A ^{[1
]}

Galeone, A ^{[1
]}

Esposito, V ^{[1
]}

Varra, M ^{[1
]}

Mayol, L ^{[1
]}

机构：

[1] Univ Naples Federico II, Dipartimento Chim Sostanze Nat, I-80131 Naples, Italy

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2002年 / 21卷 / 8-9期

关键词：

D O I：

10.1081/NCN-120015067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Homonuclear NMR techniques have been used to investigate the interactions of the minor groove binding agents distamycin A (Dist-A) and the related drug netropsin (Net) with three quadruplexes characterized by different groove widths: [d(TGGGGT)](4) (Q1), [d(GGGGTTTTGGGG)](2) (Q2), and d(GGGGTTGGGGTGTGGGGTTGGGG) (Q3). Netropsin has been found to be in a fast chemical exchange with all three kinds of quadruplexes, whereas Dist-A interacts tightly with Q1 and, at a less extent, with Q2. In order to determine the degree of selectivity of Dist-A for two- rather than four-stranded DNA, we titrated with Dist-A an equimolar solution of Q1 and the duplex d(CGCAAATTTGCG)(2) (D). This comparative H-1-NMR study allowed us to conclude that Dist-A and, consequently, Net possess higher affinity for duplex DNA.

引用

页码：535 / 545

页数：11

共 15 条

[1] SOLUTION STRUCTURE OF A PARALLEL-STRANDED TETRAPLEX FORMED BY D(TG(4)T) IN THE PRESENCE OF SODIUM-IONS BY NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY [J].