CHL1 Functions as a Nitrate Sensor in Plants

被引:1103
作者
Ho, Cheng-Hsun [1 ,2 ]
Lin, Shan-Hua
Hu, Heng-Cheng
Tsay, Yi-Fang [1 ,2 ]
机构
[1] Natl Def Med Ctr, Acad Sinica, Inst Mol Biol, Taiwan Int Grad Program, Taipei, Taiwan
[2] Natl Def Med Ctr, Acad Sinica, Grad Inst Life Sci, Taiwan Int Grad Program, Taipei, Taiwan
关键词
PROTEIN-KINASE; SACCHAROMYCES-CEREVISIAE; CALCIUM SENSORS; ROOT ARCHITECTURE; TRANSPORTER GENE; ARABIDOPSIS; REVEALS; EXPRESSION; GLUCOSE; NRT1.1;
D O I
10.1016/j.cell.2009.07.004
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ions serve as essential nutrients in higher plants and can also act as signaling molecules. Little is known about how plants sense changes in soil nutrient concentrations. Previous studies showed that T101-phosphorylated CHL1 is a high-affinity nitrate transporter, whereas T101-dephosphorylated CHL1 is a low-affinity transporter. In this study, analysis of an uptake- and sensing-decoupled mutant showed that the nitrate transporter CHL1 functions as a nitrate sensor. Primary nitrate responses in CHL1T101D and CHLT101A transgenic plants showed that phosphorylated and dephosphorylated CHL1 lead to a low- and high-level response, respectively. In vitro and in vivo studies showed that, in response to low nitrate concentrations, protein kinase CIPK23 can phosphorylate T101 of CHL1 to maintain a low- level primary response. Thus, CHL1 uses dual-affinity binding and a phosphorylation switch to sense a wide range of nitrate concentrations in the soil, thereby functioning as an ion sensor in higher plants. For a video summary of this article, see the PaperFlick file with the Supplemental Data available online.
引用
收藏
页码:1184 / 1194
页数:11
相关论文
共 38 条
[31]   THE HERBICIDE SENSITIVITY GENE CHL1 OF ARABIDOPSIS ENCODES A NITRATE-INDUCIBLE NITRATE TRANSPORTER [J].
TSAY, YF ;
SCHROEDER, JI ;
FELDMANN, KA ;
CRAWFORD, NM .
CELL, 1993, 72 (05) :705-713
[32]   Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta [J].
Waadt, Rainer ;
Schmidt, Lena K. ;
Lohse, Marc ;
Hashimoto, Kenji ;
Bock, Ralph ;
Kudla, Joerg .
PLANT JOURNAL, 2008, 56 (03) :505-516
[33]   Signaling mechanisms integrating root and shoot responses to changes in the nitrogen supply [J].
Walch-Liu, P ;
Filleur, S ;
Gan, YB ;
Forde, BG .
PHOTOSYNTHESIS RESEARCH, 2005, 83 (02) :239-250
[34]   Nitrate signalling mediated by the NRT1.1 nitrate transporter antagonises L-glutamate-induced changes in root architecture [J].
Walch-Liu, Pia ;
Forde, Brian G. .
PLANT JOURNAL, 2008, 54 (05) :820-828
[35]   Microarray analysis of the nitrate response in Arabidopsis roots and shoots reveals over 1,000 rapidly responding genes and new linkages to glucose, trehalose-6-phosphate, iron, and sulfate metabolism [J].
Wang, RC ;
Okamoto, M ;
Xing, XJ ;
Crawford, NM .
PLANT PHYSIOLOGY, 2003, 132 (02) :556-567
[36]   The Arabidopsis CHL1 protein plays a major role in high-affinity nitrate uptake [J].
Wang, RC ;
Liu, D ;
Crawford, NM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :15134-15139
[37]   A protein kinase, interacting with two calcineurin B-like proteins, regulates K+ transporter AKT1 in Arabidopsis [J].
Xu, Jiang ;
Li, Hao-Dong ;
Chen, Li-Qing ;
Wang, Yi ;
Liu, Li-Li ;
He, Liu ;
Wu, Wei-Hua .
CELL, 2006, 125 (07) :1347-1360
[38]   An Arabidopsis MADS box gene that controls nutrient-induced changes in root architecture [J].
Zhang, HM ;
Forde, BG .
SCIENCE, 1998, 279 (5349) :407-409