Filter-exchange PGSE NMR determination of cell membrane permeability

被引：104

作者：

Aslund, Ingrid ^{[1
]}

Nowacka, Agnieszka ^{[1
]}

Nilsson, Markus ^{[1
]}

Topgaard, Daniel ^{[1
]}

机构：

[1] Lund Univ, SE-22100 Lund, Sweden

来源：

JOURNAL OF MAGNETIC RESONANCE | 2009年 / 200卷 / 02期

基金：

瑞典研究理事会;

关键词：

Pulsed gradient spin echo; PGSE; Membrane permeability; Intracellular lifetime; Yeast; WATER DIFFUSION PERMEABILITY; NUCLEAR-MAGNETIC-RESONANCE; FIELD-GRADIENT NMR; SELF-DIFFUSION; SPHERICAL INTERFACE; TRANSPORT; YEAST; SPECTROSCOPY; RELAXATION; CHANNELS;

D O I：

10.1016/j.jmr.2009.07.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：291 / 295

页数：5

共 45 条

[1] Aquaporin water channels (Nobel lecture) [J].