Polynucleotide phosphorylase is a global regulator of virulence and persistency in Salmonella enterica

被引:140
作者
Clements, MO
Eriksson, S
Thompson, A
Lucchini, S
Hinton, JCD
Normark, S
Rhen, M
机构
[1] Karolinska Inst, Ctr Microbiol & Tumor Biol, S-17177 Stockholm, Sweden
[2] UCL, Wolfson Inst Biomed Res, London WC1E 6BT, England
[3] Inst Food Res, Mol Microbiol Grp, Norwich NR4 7UA, Norfolk, England
[4] Swedish Inst Infect Dis Control, S-17182 Solna, Sweden
关键词
D O I
10.1073/pnas.132047099
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
For many pathogens, the ability to regulate their replication in host cells is a key element in establishing persistency. Here, we identified a single point mutation in the gene for polynucleotide phosphorylase (PNPase) as a factor affecting bacterial invasion and intracellular replication, and which determines the alternation between acute or persistent infection in a mouse model for Salmonella enterica infection. In parallel, with microarray analysis, PNPase was found to affect the mRNA levels of a subset of virulence genes, in particular those contained in Salmonella pathogenicity islands 1 and 2. The results demonstrate a connection between PNPase and Salmonella virulence and show that alterations in PNPase activity could represent a strategy for the establishment of persistency.
引用
收藏
页码:8784 / 8789
页数:6
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