Organization of telomeric nucleosomes: atomic force microscopy imaging and theoretical modeling

被引：15

作者：

Mechelli, R

Anselmi, C

Cacchione, S

De Santis, P

Savino, M

机构：

[1] Univ Roma La Sapienza, Fdn Inst Pasteur, Fdn Cenci Bolognetti, Dipartimento Genet & Biol Mol, I-00185 Rome, Italy

[2] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

[3] CNR, Ist Biol & Patol Mol, I-00185 Rome, Italy

来源：

FEBS LETTERS | 2004年 / 566卷 / 1-3期

关键词：

telomere; nucleosomal array; atomic force microscopy; theoretical modeling; nucleosome positioning;

D O I：

10.1016/j.febslet.2004.04.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Telomeric chromatin has peculiar features with respect to bulk chromatin, which are not fully clarified to date. Nucleosomal arrays, reconstituted on fragments of human telomeric DNA and on tandemly repeated tetramers of 5S rDNA, have been investigated at single-molecule level by atomic force microscopy and Monte Carlo simulations. A satisfactory correlation emerges between experimental and theoretical internucleosomal distance distributions. However, in the case of telomeric nucleosomal arrays containing two nucleosomes, we found significant differences. Our results show that sequence features of DNA are significant in the basic chromatin organization, but are not the only determinant. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：131 / 135

页数：5

共 28 条

[1] A theoretical model for the prediction of sequence-dependent nucleosome thermodynamic stability [J].

Anselmi, C ;

Bocchinfuso, G ;

De Santis, P ;

Savino, M ;

Scipioni, A .

BIOPHYSICAL JOURNAL, 2000, 79 (02) :601-613

[2] From the sequence to the superstructural properties of DNAs [J].

Anselmi, C ;

De Santis, R ;

Paparcone, R ;

Savino, M ;

Scipioni, A .

BIOPHYSICAL CHEMISTRY, 2002, 95 (01) :23-47

[3] Dual role of DNA intrinsic curvature and flexibility in determining nucleosome stability [J].

Anselmi, C ;

Bocchinfuso, G ;

De Santis, P ;

Savino, M ;

Scipioni, A .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 286 (05) :1293-1301

[4] Condensation of rat telomere-specific nucleosomal arrays containing unusually short DNA repeats and histone H1 [J].

Bedoyan, JK ;

Lejnine, S ;

Makarov, VL ;

Langmore, JP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (31) :18485-18493

[5] Systematic search for compact structures of telomeric nucleosomes [J].

Besker, N ;

Anselmi, C ;

Paparcone, R ;

Scipioni, A ;

Savino, M ;

De Santis, P .

FEBS LETTERS, 2003, 554 (03) :369-372

[6]

Bevington R., 1969, DATA REDUCTION ERROR

[7] In vitro low propensity to form nucleosomes of four telomeric sequences [J].

Cacchione, S ;

Cerone, MA ;

Savino, M .

FEBS LETTERS, 1997, 400 (01) :37-41

[8] Acetylated nucleosome assembly on telomeric DNAs [J].

Cacchione, S ;

Rodríguez, JL ;

Mechelli, R ;

Franco, L ;

Savino, M .

BIOPHYSICAL CHEMISTRY, 2003, 104 (02) :381-392

[9] Solvent mediated interactions in the structure of the nucleosome core particle at 1.9 Å resolution [J].

Davey, CA ;

Sargent, DF ;

Luger, K ;

Maeder, AW ;

Richmond, TJ .

JOURNAL OF MOLECULAR BIOLOGY, 2002, 319 (05) :1097-1113

[10] The main role of the sequence-dependent DNA elasticity in determining the free energy of nucleosome formation on telomeric DNAs [J].

Filesi, I ;

Cacchione, S ;

De Santis, P ;

Rossetti, L ;

Savino, M .

BIOPHYSICAL CHEMISTRY, 2000, 83 (03) :223-237

← 1 2 3 →