The main role of the sequence-dependent DNA elasticity in determining the free energy of nucleosome formation on telomeric DNAs

被引：36

作者：

Filesi, I

Cacchione, S

De Santis, P

Rossetti, L

Savino, M

机构：

[1] Univ Rome La Sapienza, Fdn Cenci Bolognetti, Fdn Ist Pasteur, Dipartimento Genet & Biol Mol, I-00185 Rome, Italy

[2] Univ Rome La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

来源：

BIOPHYSICAL CHEMISTRY | 2000年 / 83卷 / 03期

关键词：

nucleosome; telomeres; theoretical prediction; sequence-dependent DNA elasticity; competitive reconstitution;

D O I：

10.1016/S0301-4622(99)00143-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a competitive reconstitution assay, we measured the free energy spent in nucleosome formation of eight telomeric DNAs, differing in sequence and/or in length. The obtained values are in satisfactorily good agreement with those derived from a theoretical model that allows the calculation of the free energy of nucleosome formation on the basis of sequence-dependent DNA elasticity, using a statistical thermodynamic approach. Both theoretical and experimental evaluations show that telomeres are characterized by the highest free energies of nucleosome formation among all the DNA sequences so far studied. The free energy of nucleosome formation varies according to the different telomeric sequences and the length of the fragments. Theoretical analysis and experimental mapping by lambda exonuclease show that telomeric nucleosomes occupy multiple positions spaced every telomeric repeat. Sequence-dependent DNA elasticity appears as the main determinant of the stability of telomeric nucleosomes and their multiple translational positioning. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：223 / 237

页数：15

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