The Expression of LEP, LEPR, IGF1 and IL10 in Obesity and the Relationship with microRNAs

被引：37

作者：

Collares, Renata Viesti A. ^{[1
]}

Salgado, Wilson, Jr. ^{[1
]}

da Cunha Tirapelli, Daniela Pretti ^{[1
,2
]}

dos Santos, Jose Sebastiao ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Surg & Anat, BR-14049 Ribeirao Preto, SP, Brazil

[2] Mol Biol Lab, Ribeirao Preto, SP, Brazil

来源：

PLOS ONE | 2014年 / 9卷 / 04期

关键词：

METABOLIC SYNDROME; ADIPOSE-TISSUE; INSULIN-RESISTANCE; GENE-EXPRESSION; SERUM LEPTIN; INTERLEUKIN-10; FAT; ADIPOCYTOKINES; ASSOCIATION; ACTIVATION;

D O I：

10.1371/journal.pone.0093512

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Obesity is a multifactorial disease, with epigenetic alterations. Have been described modifications in the expression of some microRNAs, and some proteins related to obesity. The objective was to determine and correlate, in obese patients, the gene expression of LEP, LEPR, IGF1, IL10 and of miR-27a, miR-27b, miR-143 and miR-145. RNA was extracted from biopsies of subcutaneous fat, liver and visceral fat of 15 obese subjects submitted to bariatric surgery and of 15 non-obese subjects submitted to cholecystectomy for cDNA synthesis and for RT-PCR. The microRNAs were chosen using the TargetScan software. An increased expression of LEP and IGF1 was detected in the subcutaneous fat of the obese group compared to control, while the expression of IGF1 was higher in the control group than in the obese one. MiRNA-27a had a higher expression in the omentum of the obese patients and there was also a correlation in the expression of miRNA-145 and LEPR in the omentum of this group.

引用

页数：6

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[1]

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