Purification and characterization of a proteasome from the hyperthermophilic archaeon Pyrococcus furiosus

被引：30

作者：

Bauer, MW ^{[1
]}

Bauer, SH ^{[1
]}

Kelly, RM ^{[1
]}

机构：

[1] N CAROLINA STATE UNIV,DEPT CHEM ENGN,RALEIGH,NC 27695

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1997年 / 63卷 / 03期

关键词：

D O I：

10.1128/AEM.63.3.1160-1164.1997

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A 640-kDa proteasome consisting of alpha (25-kDa) and beta (22-kDa) subunits, and with a temperature optimum of 95 degrees C, was purified from crude cell extracts of a hyperthermophilic archaeon, Pyrococcus furiosus. Although this is the fourth member of the kingdom Euryarchaeota (and the first hyperthermophile) found to contain a proteasome, none has been identified among the members of the kingdom Crenarchaeota.

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页码：1160 / 1164

页数：5

相关论文

共 38 条

[11] PYROCOCCUS-FURIOSUS SP-NOV REPRESENTS A NOVEL GENUS OF MARINE HETEROTROPHIC ARCHAEBACTERIA GROWING OPTIMALLY AT 100-DEGREES C [J].

FIALA, G ;

STETTER, KO .

ARCHIVES OF MICROBIOLOGY, 1986, 145 (01) :56-61

[12] PROTEOLYSIS, PROTEASOMES AND ANTIGEN PRESENTATION [J].

GOLDBERG, AL ;

ROCK, KL .

NATURE, 1992, 357 (6377) :375-379

[13] FUNCTIONS OF THE PROTEASOME - THE LYSIS AT THE END OF THE TUNNEL [J].

GOLDBERG, AL .

SCIENCE, 1995, 268 (5210) :522-523

[14] LOCALIZATION OF SUBUNITS IN PROTEASOMES FROM THERMOPLASMA-ACIDOPHILUM BY IMMUNOELECTRON MICROSCOPY [J].

GRZIWA, A ;

BAUMEISTER, W ;

DAHLMANN, B ;

KOPP, F .

FEBS LETTERS, 1991, 290 (1-2) :186-190

[15] DISSOCIATION AND RECONSTITUTION OF THE THERMOPLASMA PROTEASOME [J].

GRZIWA, A ;

MAACK, S ;

PUHLER, G ;

WIEGAND, G ;

BAUMEISTER, W ;

JAENICKE, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 223 (03) :1061-1067

[16] Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus [J].

Halio, SB ;

Blumentals, II ;

Short, SA ;

Merrill, BM ;

Kelly, RM .

JOURNAL OF BACTERIOLOGY, 1996, 178 (09) :2605-2612

[17]

HALIO SB, 1977, APPL ENVIRON MICROB, V63, P289

[18] THE 3-DIMENSIONAL STRUCTURE OF PROTEASOMES FROM THERMOPLASMA-ACIDOPHILUM AS DETERMINED BY ELECTRON-MICROSCOPY USING RANDOM CONICAL TILTING [J].

HEGERL, R ;

PFEIFER, G ;

PUHLER, G ;

DAHLMANN, B ;

BAUMEISTER, W .

FEBS LETTERS, 1991, 283 (01) :117-121

[19] PURIFICATION AND CHARACTERIZATION OF AN EXTREMELY THERMOSTABLE BETA-GLUCOSIDASE FROM THE HYPERTHERMOPHILIC ARCHAEON PYROCOCCUS-FURIOSUS [J].

KENGEN, SWM ;

LUESINK, EJ ;

STAMS, AJM ;

ZEHNDER, AJB .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 213 (01) :305-312

[20] CRYSTAL-STRUCTURE OF THE 20S PROTEASOME FROM THE ARCHAEON T-ACIDOPHILUM AT 3.4-ANGSTROM RESOLUTION [J].

LOWE, J ;

STOCK, D ;

JAP, R ;

ZWICKL, P ;

BAUMEISTER, W ;

HUBER, R .

SCIENCE, 1995, 268 (5210) :533-539

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