Epstein-Barr virus vectors for gene delivery to B lymphocytes

被引：44

作者：

Robertson, ES

Ooka, T

Kieff, ED

机构：

[1] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOL GENET,VIROL PROGRAM,BOSTON,MA 02115

[2] UNIV LYON 1,FAC MED R LAENNEC,MOL VIROL LAB,CNRS UMR30,F-69372 LYON 08,FRANCE

[3] HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 21期

关键词：

D O I：

10.1073/pnas.93.21.11334

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Basic research in Epstein-Barr virus (EBV) molecular genetics has provided means to maintain episomes in human cells, to efficiently deliver episomes with up to 150 kbp of heterologous DNA to human B lymphocytes, and to immortalize human B lymphocytes with EBV recombinants that can maintain up to 120 kbp of heterologous DNA. Episome maintenance requires an EBV nuclear protein, EBNA1, whereas immortalization of cells with EBV recombinants requires EBNA1, EBNA2, EBNA3A, EBNA3C, EBNALP, and LMP1. EBV-derived vectors are useful for experimental genetic reconstitution in B lymphocytes, a cell type frequently used in establishing repositories of human genetic deficiencies. The ability of EBV-infected cells to establish a balanced state of persistence in normal humans raises the possibility that cells infected with EBV recombinants could be useful for genetic reconstitution, in vivo.

引用

页码：11334 / 11340

页数：7

共 40 条

[31] A VECTOR THAT REPLICATES AS A PLASMID AND CAN BE EFFICIENTLY SELECTED IN LYMPHOBLASTS-B TRANSFORMED BY EPSTEIN-BARR VIRUS [J].