Quantitative analysis of pro-inflammatory cytokine mRNA expression in Theileria annulata-infected cell lines derived from resistant and susceptible cattle

被引:50
作者
McGuire, K [1 ]
Manuja, A
Russell, GC
Springbett, A
Craigmile, SC
Nichani, AK
Malhotra, DV
Glass, EJ
机构
[1] Roslin Inst, Dept Genom & Bioinformat, Roslin EH25 9PS, Midlothian, Scotland
[2] CCS Haryana Agr Univ, Dept Vet Epidemiol & Prevent Med, Hisar 125004, Haryana, India
基金
英国惠康基金; 英国生物技术与生命科学研究理事会;
关键词
Theileria annulata; real time RT-PCR; pro-inflammatory cytokines; resistance;
D O I
10.1016/j.vetimm.2004.01.003
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The pathogenic mechanisms involved in tropical theileriosis, caused by the fick-borne protozoan parasite Theileria annulata, are unclear. Pathology is associated with the schizont stage of the parasite, which resides within bovine macrophages. Breed-specific differences in pathology have been observed in cattle, several Bos indicus breeds are relatively resistant to tropical theileriosis whilst Bos taurus cattle are highly susceptible. Infected cells express pro-inflammatory cytokines and it has been hypothesized that these cytokines play a major role in the pathology of the disease. Therefore, using quantitative RT-PCR we investigated the expression of the key candidates, interleukin 1 beta (IL-1beta), IL-6 and tumour necrosis factor alpha (TNF-alpha), in T annulata low passage infected cell lines derived ex vivo from experimental infection of resistant and susceptible cattle. mRNA for each cytokine was detected in all cell lines investigated at levels higher than those observed in resting monocytes. However, the analyses did not identify any breed-specific differences. Therefore, these results are not consistent with the hypothesis that differential regulation of infected cell derived pro-inflammatory cytokines (IL-1beta, IL-6 and TNF-alpha) accounts for the breed-related differences in resistance and susceptibility to T annulata infection. Other, currently unknown mechanisms may be of greater importance. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:87 / 98
页数:12
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