Single-Particle Cryo-Electron Microscopy (Cryo-EM): Progress, Challenges, and Perspectives for Further Improvement

被引:16
作者
Agard, David [1 ,2 ]
Cheng, Yifan [2 ]
Glaeser, Robert M. [3 ]
Subramaniam, Sriram [4 ]
机构
[1] Univ Calif San Francisco, HHMI, San Francisco, CA 94158 USA
[2] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA
[3] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA
[4] NCI, Lab Cell Biol, Ctr Canc Res, NIH, Bethesda, MD 20892 USA
来源
ADVANCES IN IMAGING AND ELECTRON PHYSICS, VOL 185 | 2014年 / 185卷
关键词
BEAM-INDUCED MOTION; ELECTRON CRYOMICROSCOPY; 20S PROTEASOME; EWALD SPHERE; RESOLUTION; MACROMOLECULES; IMAGES; CLASSIFICATION; CONTRAST; ICE;
D O I
10.1016/B978-0-12-800144-8.00002-1
中图分类号
O59 [应用物理学];
学科分类号
摘要
引用
收藏
页码:113 / 137
页数:25
相关论文
共 45 条
[1]   Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles [J].
Bai, Xiao-chen ;
Fernandez, Israel S. ;
McMullan, Greg ;
Scheres, Sjors H. W. .
ELIFE, 2013, 2
[2]   The resolution dependence of optimal exposures in liquid nitrogen temperature electron cryomicroscopy of catalase crystals [J].
Baker, Lindsay A. ;
Smith, Eric A. ;
Bueler, Stephanie A. ;
Rubinstein, John L. .
JOURNAL OF STRUCTURAL BIOLOGY, 2010, 169 (03) :431-437
[3]   Beam-induced motion of vitrified specimen on holey carbon film [J].
Brilot, Axel F. ;
Chen, James Z. ;
Cheng, Anchi ;
Pan, Junhua ;
Harrison, Stephen C. ;
Potter, Clinton S. ;
Carragher, Bridget ;
Henderson, Richard ;
Grigorieff, Nikolaus .
JOURNAL OF STRUCTURAL BIOLOGY, 2012, 177 (03) :630-637
[4]   Movies of Ice-Embedded Particles Enhance Resolution in Electron Cryo-Microscopy [J].
Campbell, Melody G. ;
Cheng, Anchi ;
Brilot, Axel F. ;
Moeller, Arne ;
Lyumkis, Dmitry ;
Veesler, David ;
Pan, Junhua ;
Harrison, Stephen C. ;
Potter, Clinton S. ;
Carragher, Bridget ;
Grigorieff, Nikolaus .
STRUCTURE, 2012, 20 (11) :1823-1828
[5]   TRPV1 structures in distinct conformations reveal activation mechanisms [J].
Cao, Erhu ;
Liao, Maofu ;
Cheng, Yifan ;
Julius, David .
NATURE, 2013, 504 (7478) :113-+
[6]   3 DIMENSIONAL RECONSTRUCTIONS OF SPHERICAL VIRUSES BY FOURIER SYNTHESIS FROM ELECTRON MICROGRAPHS [J].
CROWTHER, RA ;
AMOS, LA ;
FINCH, JT ;
DEROSIER, DJ ;
KLUG, A .
NATURE, 1970, 226 (5244) :421-&
[7]   Correction of high-resolution data for curvature of the Ewald sphere [J].
DeRosier, DJ .
ULTRAMICROSCOPY, 2000, 81 (02) :83-98
[8]   Restoration of weak phase-contrast images recorded with a high degree of defocus: The "twin image" problem associated with CTF correction [J].
Downing, Kenneth H. ;
Glaeser, Robert M. .
ULTRAMICROSCOPY, 2008, 108 (09) :921-928
[9]  
Frank J., 2006, Three-dimensional electron microscopy of macromolecular assemblies: visualization of biological molecules in their native state
[10]   Story in a samplethe potential (and limitations) of cryo-electron microscopy applied to molecular machines [J].
Frank, Joachim .
BIOPOLYMERS, 2013, 99 (11) :832-836