An evaluation of factors affecting the efficiency of Agrobacterium-mediated transformation of Citrus paradisi (Macf.) and production of transgenic plants containing carotenoid biosynthetic genes

被引：51

作者：

Costa, MGC

Otoni, WC

Moore, GA ^{[1
]}

机构：

[1] Univ Florida, Dept Hort Sci, Inst Food & Agr Sci, Gainesville, FL 32611 USA

[2] Univ Fed Vicosa, Dept Plant Biol, BR-36571000 Vicosa, MG, Brazil

来源：

PLANT CELL REPORTS | 2002年 / 21卷 / 04期

关键词：

Citrus paradisi; grapefruit; genetic transformation; carotenoid;

D O I：

10.1007/s00299-002-0533-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An improved protocol for Agrobacterium-mediated transformation of Duncan grapefruit (Citrus paradisi Macf.) epicotyl explants was developed by examining the effects of six different factors on the efficiency of transformation and combining the best treatment for each factor. The preculturing of explants and the composition of the cocultivation medium were the factors that most influenccd transformation efficiency. The optimized protocol was successfully employed in the production of transgenic grapefruit plants containing the carotenoid biosynthetic genes phytoene synthase, phytoene desaturase, or lycopene-beta-cyclase under constitutive expression. With an eventual goal of metabolically engineering grapefruit with multiple genes, hygromycin as a selectable marker and. BIBAC as a transformation vector for large pieces of DNA were also tested.

引用

页码：365 / 373

页数：9

共 36 条

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