Medium-chain dehydrogenases/reductases (MDR) -: Family characterizations including genome comparisons and active site modelling

被引:136
作者
Nordling, E
Jörnvall, H
Persson, B [1 ]
机构
[1] Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden
[2] Karolinska Inst, Stockholm Bioinformat Ctr, S-17177 Stockholm, Sweden
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 2002年 / 269卷 / 17期
关键词
medium-chain dehydrogenases/reductases; genome comparisons; polyol dehydrogenase; cinnamyl alcohol dehydrogenase; quinone oxidoreductase;
D O I
10.1046/j.1432-1033.2002.03114.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Completed eukaryotic genomes were screened for medium-chain dehydrogenases/reductases (MDR). In the human genome, 23 MDR forms were found, a number that probably will increase, because the genome is not yet fully interpreted. Partial sequences already indicate that at least three further members exist. Within the MDR superfamily, at least eight families were distinguished. Three families are formed by dimeric alcohol dehydrogenases (ADH; originally detected in animals/plants), cinnamyl alcohol dehydrogenases (originally detected in plants) and tetrameric alcohol dehydrogenases (originally detected in yeast). Three further families are centred around forms initially detected as mitochondrial respiratory function proteins, acetyl-CoA reductases of fatty acid synthases, and leukotriene B4 dehydrogenases. The two remaining families with polyol dehydrogenases (originally detected as sorbitol dehydrogenase) and quinone reductases (originally detected as zeta-crystallin) are also distinct but with variable sequences. The most abundant families in the human genome are the dimeric ADH forms and the quinone oxidoreductases. The eukaryotic patterns are different from those of Escherichia coli. The different families were further evaluated by molecular modelling of their active sites as to geometry, hydrophobicity and volume of substrate-binding pockets. Finally, sequence patterns were derived that are diagnostic for the different families and can be used in genome annotations.
引用
收藏
页码:4267 / 4276
页数:10
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