Phenotypic conversion of drug-resistant bacteria to drug sensitivity

被引：66

作者：

GuerrierTakada, C ^{[1
]}

Salavati, R ^{[1
]}

Altman, S ^{[1
]}

机构：

[1] YALE UNIV,DEPT BIOL,NEW HAVEN,CT 06520

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 16期

关键词：

bacterial drug resistance; external guide sequences; RNase P;

D O I：

10.1073/pnas.94.16.8468

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plasmids that contain synthetic genes coding for small oligoribonucleotides called external guide sequences (EGSs) have been introduced into strains of Escherichia coli harboring antibiotic resistance genes. The EGSs direct RNase P to cleave the mRNAs transcribed from these genes thereby converting the phenotype of drug-resistant cells to drug sensitivity, Increasing the EGS-to-target mRNA ratio by changing gene copy number or the number of EGSs complementary to different target sites enhances the efficiency of the conversion process, We demonstrate a general method for the efficient phenotypic conversion of drug-resistant bacterial cultures.

引用

页码：8468 / 8472

页数：5

共 24 条

[11] TARGETED CLEAVAGE OF MESSENGER-RNA INVITRO BY RNASE-P FROM ESCHERICHIA-COLI [J].