Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor

被引：134

作者：

Heim, Nicola

Garaschuk, Olga

Friedrich, Michael W.

Mank, Marco

Milos, Ruxandra I.

Kovalchuk, Yury

Konnerth, Arthur

Griesbeck, Oliver

机构：

[1] Max Planck Inst Neurobiol, D-82152 Martinsried, Germany

[2] Tech Univ Munich, Inst Neurosci, D-80802 Munich, Germany

来源：

NATURE METHODS | 2007年 / 4卷 / 02期

关键词：

D O I：

10.1038/nmeth1009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescent Ca2+ indicator proteins (FCIPs) are attractive tools for studying Ca2+ dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca2+ sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca2+ imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.

引用

收藏

页码：127 / 129

页数：3

相关论文

共 13 条

[1] Phosphorylation of calmodulin - Functional implications [J].

Benaim, G ;

Villalobo, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2002, 269 (15) :3619-3631

[2] Overexpression of growth-associated proteins in the neurons of adult transgenic mice [J].

Caroni, P .

JOURNAL OF NEUROSCIENCE METHODS, 1997, 71 (01) :3-9

[3] Activation of cerebellar parallel fibers monitored in transgenic mice expressing a fluorescent Ca2+ indicator protein [J].

Díez-García, J ;

Matsushita, S ;

Mutoh, H ;

Nakai, J ;

Ohkura, M ;

Yokoyama, J ;

Dimitrov, D ;

Knöpfel, T .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2005, 22 (03) :627-635

[4] Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo [J].

Garaschuk, Olga ;

Milos, Ruxandra-Iulia ;

Konnerth, Arthur .

NATURE PROTOCOLS, 2006, 1 (01) :380-386

[5] Reducing the environmental sensitivity of yellow fluorescent protein - Mechanism and applications [J].

Griesbeck, O ;

Baird, GS ;

Campbell, RE ;

Zacharias, DA ;

Tsien, RY .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (31) :29188-29194

[6] Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control [J].

Hasan, MT ;

Friedrich, RW ;

Euler, T ;

Larkum, ME ;

Giese, G ;

Both, M ;

Duebel, J ;

Waters, J ;

Bujard, H ;

Griesbeck, O ;

Tsien, RY ;

Nagai, T ;

Miyawaki, A ;

Denk, W .

PLOS BIOLOGY, 2004, 2 (06) :763-775

[7] Genetically encoded indicators of cellular calcium dynamics based on troponin C and green fluorescent protein [J].

Heim, N ;

Griesbeck, O .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (14) :14280-14286

[8] Apocalmodulin [J].

Jurado, LA ;

Chockalingam, PS ;

Jarrett, HW .

PHYSIOLOGICAL REVIEWS, 1999, 79 (03) :661-682

[9] Innovations in the imaging of brain functions using fluorescent proteins [J].

Miyawaki, A .

NEURON, 2005, 48 (02) :189-199

[10] Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins [J].

Nagai, T ;

Yamada, S ;

Tominaga, T ;

Ichikawa, M ;

Miyawaki, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (29) :10554-10559