A new approach for preparation of essential oil, followed by chlorogenic acid and hyperoside with microwave-assisted simultaneous distillation and dual extraction (MSDDE) from Vaccinium uliginosum leaves

Volatile essential oil, non-volatile chlorogenic acid and hyperoside were effectively extracted and isolated from leaves of Vaccinium uliginosum by microwave-assisted simultaneous distillation and dual extraction. After optimization by response surface methodology, satisfactory yields for the three above-mentioned components were obtained under the following optimum conditions: liquid-solid ratio of 20 mL/g, microwave irradiation time of 26 min, and microwave irradiation power of 389W. Compared with conventional methods, gas chromatography-mass spectrometry analysis of the essential oil showed that this method did not produce substantial changes in its chemical composition. However, the proposed method produced higher yields of volatile essential oil, non-volatile chlorogenic acid and hyperoside in a shorter time than conventional methods. Adsorption and desorption experiments with HPD 100B macroporous resin and ethanol/water solutions of various volume fractions led to optimization of the ethanol volume fraction for effective isolation of chlorogenic acid and hyperoside from the aqueous extract. (C) 2015 Elsevier B.V. All rights reserved.