Rapid and high-throughput purification of salvianolic acid B from Salvia miltiorrhiza Bunge by high-performance counter-current chromatography

被引：37

作者：

Zhang, Min ^{[1
,2
]}

Ignatova, Svetlana ^{[3
]}

Liang, Qionglin ^{[1
]}

Jun, Frank Wu ^{[4
]}

Sutherland, Ian ^{[3
]}

Wang, Yiming ^{[1
]}

Luo, Guoan ^{[1
,2
]}

机构：

[1] Tsinghua Univ, Inst Biomed, Beijing 100084, Peoples R China

[2] E China Univ Sci & Technol, Modern Engn Ctr Tradit Chinese Med, Sch Pharm, Shanghai 200237, Peoples R China

[3] Brunel Univ, Brunel Inst Bioengn, Uxbridge UB8 3PH, Middx, England

[4] XiangCam TCM Res Ctr Ltd, Unit 16, Cambridge CB22 3AT, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 18期

关键词：

Counter-current chromatography; Salvianolic acid B; Salvia miltiorrhiza Bunge; High throughput; Process efficiency; LC-MS-MS; SEPARATION; IDENTIFICATION; EXTRACT; SYSTEM;

D O I：

10.1016/j.chroma.2009.02.067

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

A large-scale purification of salvianolic acid B from Salvia miltiorrhiza Bunge is presented. The method development began with selection of the solvent system, then optimization of the operating parameters and ended up with linear scale-up from an analytical to a preparative instrument. Three factors were used for method optimization and scale-up estimation: purity, process throughput and process efficiency. Preparation was achieved using a two-phase solvent system comprising hexane-ethyl acetate-methanol-acetic acid-water (1:5:1.5:0.00596:5, v/v). This preparation yielded 475 mg of salvianolic acid B with a purity of 96.1% from 1.5g of crude extract. The process throughput of crude was 2.23 g/h while process efficiency per gram of target compound was 0.769 g/h. Two factors-process environmental risk factor and process evaluation factor were used for evaluation of the separation process. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：3869 / 3873

页数：5

共 22 条

[1]

Separation and identification of water-soluble salvianolic acids from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography and ESI-MS analysis [J].