Simple approach to the development of a CCC solvent selection protocol suitable for automation

被引：61

作者：

Garrard, IJ ^{[1
]}

机构：

[1] Brunel Univ, Brunel Inst Bioengn, Uxbridge UB8 3PH, Middx, England

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2005年 / 28卷 / 12-13期

关键词：

countercurrent chromatography; CCC; solvent selection;

D O I：

10.1081/JLC-200063571

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

One of the biggest problems with countercurrent chromatography, when it comes to its practical application in an industrial environment, is developing a protocol and corresponding solvent system for its operation. This paper critically reviews previous proposals for solvent selection and suggests a scheme that is suitable for use by an inexperienced user and for automation using a modern liquid handling robot.

引用

页码：1923 / 1935

页数：13

共 10 条

[1]

BERTHOD A, 1991, CHROMATOGR ANAL FEB, P12

[2]

Effect of Digitalis lanata matrix composition on the lanatoside C partition coefficient and its consequence on rotation locular countercurrent chromatography efficiency [J].

Braga, FC ;

Kreis, W ;

de Oliveira, AB .

JOURNAL OF CHROMATOGRAPHY A, 1998, 822 (01) :37-44

[3]

CamachoFrias E, 1996, ANALUSIS, V24, P159

[4]

Counter-current chromatography: instrumentation, solvent selection and some recent applications to natural product purification [J].

Foucault, AP ;

Chevolot, L .

JOURNAL OF CHROMATOGRAPHY A, 1998, 808 (1-2) :3-22

[5]

FOUCAULT AP, 1994, CHROMATOGRAPHIC SCI, V68, P71

[6]

Gunawardana G, 1999, CHROMATOGR SCI SERIE, V82, P249

[7]

COUNTERCURRENT CHROMATOGRAPHY AS A PREPARATIVE TOOL - APPLICATIONS AND PERSPECTIVES [J].

MARSTON, A ;

HOSTETTMANN, K .

JOURNAL OF CHROMATOGRAPHY A, 1994, 658 (02) :315-341

[8]

MARTIN DG, 1986, AM LAB OCT, P21

[9]

SYSTEMATIC SEARCH FOR SUITABLE 2-PHASE SOLVENT SYSTEMS FOR HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY [J].

OKA, F ;

OKA, H ;

ITO, Y .

JOURNAL OF CHROMATOGRAPHY, 1991, 538 (01) :99-108

[10]

THIEBAUT D, 1993, LC GC, V6, P154

← 1 →