23-Hydroxybetulinic acid-mediated apoptosis is accompanied by decreases in bcl-2 expression and telomerase activity in HL-60 Cells

被引：62

作者：

Ji, ZN

Ye, WC

Liu, GG

Hsiao, WLW ^{[1
]}

机构：

[1] Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China

[2] China Pharmaceut Univ, Dept Phytochem, Nanjing 210009, Peoples R China

[3] China Pharmaceut Univ, Dept Pharmacol, Nanjing 210009, Peoples R China

来源：

LIFE SCIENCES | 2002年 / 72卷 / 01期

基金：

中国国家自然科学基金;

关键词：

23-Hydroxybetulinic acid; telomerase; apoptosis; bcl-2; gene; HL-60; cells;

D O I：

10.1016/S0024-3205(02)02176-8

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

23-Hydroxybetulinic acid, a derivative of betulinic acid, was investigated for its apoptotic effect and the associated telomerase activity in human leukemia HL-60 cells. Apoptosis and bcl-2 were determined by flow cytometry analysis. A PCR-based telomeric repeat amplification protocol assay was used to detect telomerase activity. Results showed that 23-hydroxybetulinic acid induced growth arrest and apoptotic cell death in HL-60 cells. The apoptotic events were associated with concurrent down-regulation of bcl-2 and the telomerase activity. Our data suggest that 23-hydroxybetulinic acid may be a potential cytotoxic agent for treatment of cancer. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：1 / 9

页数：9

共 20 条

[1] FLOW CYTOMETRIC DETECTION OF THE MITOCHONDRIAL BCL-2 PROTEIN IN NORMAL AND NEOPLASTIC HUMAN LYMPHOID-CELLS [J].

AIELLO, A ;

DELIA, D ;

BORRELLO, MG ;

BIASSONI, D ;

GIARDINI, R ;

FONTANELLA, E ;

PEZZELLA, F ;

PULFORD, K ;

PIEROTTI, M ;

DELLAPORTA, G .

CYTOMETRY, 1992, 13 (05) :502-509

[2]

ALLEY MC, 1988, CANCER RES, V48, P589

[3] FEATURES OF APOPTOTIC CELLS MEASURED BY FLOW-CYTOMETRY [J].

DARZYNKIEWICZ, Z ;

BRUNO, S ;

DELBINO, G ;

GORCZYCA, W ;

HOTZ, MA ;

LASSOTA, P ;

TRAGANOS, F .

CYTOMETRY, 1992, 13 (08) :795-808

[4] Anti-apoptotic role of telomerase in pheochromocytoma cells [J].

Fu, WM ;

Begley, JG ;

Killen, MW ;

Mattson, MP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (11) :7264-7271

[5]

Fulda S, 2000, MED PEDIATR ONCOL, V35, P616, DOI 10.1002/1096-911X(20001201)35:6<616::AID-MPO27>3.0.CO

[6]

2-N

[7] Inhibition of human telomerase in immortal human cells leads to progressive telomere shortening and cell death [J].

Herbert, BS ;

Pitts, AE ;

Baker, SI ;

Hamilton, SE ;

Wright, WE ;

Shay, JW ;

Corey, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (25) :14276-14281

[8] Telomerase activity in human breast tumors [J].

Hiyama, E ;

Gollahon, L ;

Kataoka, T ;

Kuroi, K ;

Yokoyama, T ;

Gazdar, AF ;

Hiyama, K ;

Piatyszek, MA ;

Shay, JW .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1996, 88 (02) :116-122

[9]

Jiangsu New Medical College, 1977, DICT TRAD CHIN MED, P704

[10] Synthesis of betulinic acid derivatives with activity against human melanoma [J].

Kim, DSHL ;

Pezzuto, JM ;

Pisha, E .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1998, 8 (13) :1707-1712

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