Expression and down-regulation of cytochrome P450 genes of the CYP4 family by ecdysteroid agonists in Spodoptera littoralis and Drosophila melanogaster

被引：25

作者：

Davies, Lyndsay ^{[1
]}

Williams, Daryl R. ^{[1
]}

Aguiar-Santana, Ione A. ^{[1
]}

Pedersen, Julia ^{[1
]}

Turner, Philip C. ^{[1
]}

Rees, Huw H. ^{[1
]}

机构：

[1] Univ Liverpool, Sch Biol Sci, Cell Regulat & Signalling Div, Liverpool L69 7ZB, Merseyside, England

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2006年 / 36卷 / 10期

基金：

英国生物技术与生命科学研究理事会;

关键词：

cytochrome P450; CYP4; genes; insects; ecdysteroid agonists; gene expression;

D O I：

10.1016/j.ibmb.2006.08.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The function of CYP4 genes in insects is poorly understood. Some CYP genes are up-regulated by ecdysteroids and a number of Cyp4 genes in Drosophila melanogaster have been shown by microarray to be down-regulated when the ecdysteroid titre is high, suggesting hormonal regulation. Here, we report the utilization of certain cloned CYP4 cDNAs/fragments to probe their developmental/tissue expression in the Lepidopteran, Spodoptera littoralis, including the effects of ecdysteroid receptor agonists (bis-acyl hydrazines). CYP4L8 is expressed essentially throughout the final larval instar of S. littoralis and, together with CYP4M12, is down-regulated by agonist. Furthermore, expression of these genes occurs in midgut, but is undetectable in brain, fat body, and integument. Similarly, in D. nielanogaster, Cyp4ac1, Cyp4ac3, Cyp4ad1 and Cyp4d1 gene expression is drastically down-regulated by ecdysteroid agonist. The significance of the results is discussed in relation to the plausible functions of the CYP4 genes in Lepidoptera and mechanisms of down-regulation. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：801 / 807

页数：7

共 37 条

[1]

[Anonymous], 1985, COMPREHENSIVE INSECT

[2] Molting cycle-dependent expression of CYP4C15, a cytochrome P450 enzyme putatively involved in ecdysteroidogenesis in the crayfish, Orconectes limosus [J].