Csm3, Tof1, and Mrc1 Form a Heterotrimeric Mediator Complex That Associates with DNA Replication Forks

被引：109

作者：

Bando, Masashige ^{[1
]}

Katou, Yuki ^{[1
]}

Komata, Makiko ^{[1
]}

Tanaka, Hirokazu ^{[1
]}

Itoh, Takehiko ^{[1
]}

Sutani, Takashi ^{[1
]}

Shirahige, Katsuhiko ^{[1
]}

机构：

[1] Tokyo Inst Technol, Dept Biol Sci, Lab Chromosome Struct & Funct, Grad Sch Biosci & Biotechnol,Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2009年 / 284卷 / 49期

关键词：

S-PHASE CHECKPOINT; SACCHAROMYCES-CEREVISIAE; EUKARYOTIC DNA; PROGRESSION; HELICASE; STRESS; RAD53; MCM; COMPONENTS; RESPONSES;

D O I：

10.1074/jbc.M109.065730

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mrc1(mediator of replication checkpoint), Tof1 (topoisomerase I interacting factor), and Csm3 (chromosome segregation in meiosis) are checkpoint-mediator proteins that function during DNA replication and activate the effector kinase Rad53. We reported previously that Mrc1 and Tof1 are constituents of the replication machinery and that both proteins are required for the proper arrest and stabilization of replication forks in the presence of hydroxyurea. In our current study, we show that Csm3 is a component of moving replication forks and that both Tof1 and Csm3 are specifically required for the association of Mrc1 with these structures. In contrast, the deletion of mrc1 did not affect the association of Tof1 and Csm3 with the replication fork complex. In agreement with previous observations in yeast cells, the results of a baculovirus coexpression system showed that these three proteins interact directly with each other to form a mediator complex in the absence of replication forks.

引用

页码：34355 / 34365

页数：11

共 30 条

[11] Characterization of a novel CDC gene (ORC1) partly homologous to CDC6 of Saccharomyces cerevisiae [J].