Biomedical applications of X-ray absorption and vibrational spectroscopic microscopies in obtaining structural information from complex systems

被引：29

作者：

Aitken, Jade B. ^{[1
]}

Carter, Elizabeth A. ^{[1
]}

Eastgate, Harold ^{[2
]}

Hackett, Mark J. ^{[1
]}

Harris, Hugh H. ^{[1
]}

Levina, Aviva ^{[1
]}

Lee, Yao-Chang ^{[3
]}

Chen, Ching-Iue ^{[3
]}

Lai, Barry ^{[4
]}

Vogt, Stefan ^{[4
]}

Lay, Peter A. ^{[1
]}

机构：

[1] Univ Sydney, Sch Chem, Sydney, NSW 2006, Australia

[2] Eastmac Pty Ltd, Knoxfield, Vic 3180, Australia

[3] Natl Synchrotron Radiat Res Ctr, Hsinchu 30076, Taiwan

[4] Argonne Natl Lab, Xray Sci Div, Argonne, IL 60439 USA

来源：

RADIATION PHYSICS AND CHEMISTRY | 2010年 / 79卷 / 02期

基金：

美国国家卫生研究院; 澳大利亚研究理事会;

关键词：

X-ray absorption spectroscopy; X-ray microprobe; FTIR microprobe; Raman microprobes; Cells; Tissues; LIGAND BOND LENGTHS; HAMSTER LUNG-CELLS; 3-DIMENSIONAL STRUCTURE; BIOLOGICAL MEDIA; DENTAL-AMALGAM; FINE-STRUCTURE; FLUORESCENCE; SCATTERING; MICROPROBE; RESOLUTION;

D O I：

10.1016/j.radphyschem.2009.03.068

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Protein crystallography and NMR spectroscopy took decades to emerge as routine techniques in structural biology. X-ray absorption spectroscopy now has reached a similar stage of maturity for obtaining complementary local structural information around metals in metalloproteins. However, the relatively recent emergence of X-ray and vibrational spectroscopic microprobes that build on these techniques has enabled the structural information obtained from the "mature" techniques on isolated biomolecules to be translated into in situ structural information from inhomogeneous complex systems, such as whole cells and tissues. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：176 / 184

页数：9