Cytochromes P450, P420 & mixed-function oxidases as biomarkers of polychlorinated biphenyl (PCB) exposure in harbour seals (Phoca vitulina)

被引：25

作者：

Troisi, GM

Mason, CF

机构：

[1] Dept. of Biol. and Chemical Sciences, University of Essex, Colchester, CO4 3SQ, Essex

来源：

CHEMOSPHERE | 1997年 / 35卷 / 09期

关键词：

D O I：

10.1016/S0045-6535(97)00268-3

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Hepatic microsomal cytochrome P450, EROD and ECOD activity were investigated as biomarkers of PCB exposure in harbour seals (Phoca vitulina). Due to the difficulty of obtaining undegraded seal liver samples, standard spectrophotometric methodology was adapted to investigate P420 (degraded P450) as a PCB biomarker with partially degraded samples. Total PCB burdens in both blubber and liver had positive correlations with P450, P420 and MFO activity levels. The use of P420 biomarkers in this study supports the inclusion of samples from by-caught marine mammals for future biomonitoring studies. P450 isozymes CYMA (P4501A) and CYP2B (P4502B) in conjunction with MFO activity were investigated as ''specific'' biomarkers of PCB exposure. They were found to reliably reflect levels of [MC] and [PB]-type PCB exposure in harbour seal liver. (C) 1997 Elsevier Science Ltd.

引用

页码：1933 / 1946

页数：14

共 40 条

[1] MIXED-FUNCTION OXIDASE ACTIVITY IN THE HARBOR SEAL (PHOCA-VITULINA) FROM SABLE IS, NS [J].