Some observations on the copper tolerance of bacterial communities determined by the (3H)-thymidine incorporation method in heavy metal polluted humus

被引：9

作者：

Kiikkilä, O

Pennanen, T

Pietikäinen, J

Hurme, KR

Fritze, H

机构：

[1] Finnish Forest Res Inst, Vantaa Res Ctr, FIN-01301 Vantaa, Finland

[2] Univ Helsinki, Isotope Lab, FIN-00014 Helsinki, Finland

来源：

SOIL BIOLOGY & BIOCHEMISTRY | 2000年 / 32卷 / 06期

基金：

芬兰科学院;

关键词：

copper; heavy metals; microbial activity; thymidine;

D O I：

10.1016/S0038-0717(99)00217-5

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

Changes in pH after filtration of bacterial suspensions are important when applying the radioactive thymidine incorporation method to heavy-metal polluted soils with low microbial activity. In the original method (Baath 1992; Soil Biology and Biochemistry 24, 1157-1165) the blended and centrifuged suspension was filtered through glass wool to remove humus particles from the suspension. When we filtered the bacterial suspension through glass wool the pH increased by 2 units and the thymidine incorporation rate decreased. This made the community copper tolerance measurement ambiguous. When using soil samples with very low activity, we recommend the use of acid-washed glass wool or polyester net filtration which eliminates changes in pH. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：883 / 885

页数：3