Endogenous Hydrogen Peroxide Plays a Positive Role in the Upregulation of Heme Oxygenase and Acclimation to Oxidative Stress in Wheat Seedling Leaves

被引:65
作者
Chen, Xiao-Yue [1 ,2 ]
Ding, Xiao [1 ,2 ]
Xu, Sheng [1 ,2 ]
Wang, Ren [3 ]
Xuan, Wei [1 ,2 ]
Cao, Ze-Yu [1 ,2 ]
Chen, Jian [1 ,2 ]
Wu, Hong-Hong [1 ,2 ]
Ye, Mao-Bing [1 ,2 ]
Shen, Wen-Biao [1 ,2 ]
机构
[1] Centrifuge Tech Nanjing Agr Univ, Cooperat Demonstrat Lab, Coll Life Sci, Nanjing 210095, Peoples R China
[2] Nanjing Agr Univ, Beckman Coulter Ltd Co, Nanjing 210095, Peoples R China
[3] Chinese Acad Sci Mem Sun Yat Sen, Jiangsu Prov Key Lab Plant Ex Situ Conservat, Nanjing 210014, Peoples R China
关键词
acclimation to oxidative stress; endogenous hydrogen peroxide; heme oxygenase; signal transduction; Triticum; PHYTOCHROME CHROMOPHORE BIOSYNTHESIS; NITRIC-OXIDE; CARBON-MONOXIDE; ANTIOXIDANT DEFENSE; GENE-EXPRESSION; MEDICAGO-SATIVA; SOYBEAN LEAVES; DISEASE RESISTANCE; SALT TOLERANCE; NADPH OXIDASE;
D O I
10.1111/j.1744-7909.2009.00869.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pretreatment of lower H2O2 doses (0.05, 0.5 and 5 mM) for 24 h was able to dose-dependently attenuate lipid peroxidation in wheat seedling leaves mediated by further oxidative damage elicited by higher dose of H2O2 (150 mM) for 6 h, with 0.5 mM H2O2 being the most effective concentrations. Further results illustrated that 0.5 mM H2O2 pretreatment triggered the biphasic production of H2O2 during a 24 h period. We also noticed that only peak I (0.25 h) rather than peak II (4 h) was approximately consistent with the enhancement of heme oxygenase (HO) activity, HO-1 gene expression. Meanwhile, enhanced superoxide dismutase (SOD) activity, Mn-SOD and Cu,Zn-SOD transcripts might be a potential source of peak I of endogenous H2O2. Further results confirmed that 0.5 mM H2O2 treatment for 0.5 h was able to upregulate HO gene expression, which was detected by enzyme activity determination, semi-quantitative reverse transcription-polymerase chain reaction and western blotting. Meanwhile, the application of N,N'-dimethylthiourea, a trap for endogenous H2O2, not only blocked the upregulation of HO, but also reversed the corresponding oxidation attenuation. Together, the above results suggest that endogenous H2O2 production (peak I) plays a positive role in the induction of HO by enhancing its mRNA level and protein expression, thus leading to the acclimation to oxidative stress.
引用
收藏
页码:951 / 960
页数:10
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