Pacing two T cell epitopes:: A degree of randomness in the primary response is lost upon secondary immunization

被引:11
作者
Bousso, P [1 ]
Lemaître, F
Bilsborough, J
Kourilsky, P
机构
[1] Inst Pasteur, U277, Unite Biol Mol Gene, INSERM, F-75724 Paris, France
[2] Catholic Univ Louvain, Ludwig Inst Canc Res, Brussels Branch, Brussels, Belgium
[3] Catholic Univ Louvain, Cellular Genet Unit, Brussels, Belgium
关键词
D O I
10.4049/jimmunol.165.2.760
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have analyzed the hierarchy of epitope-specific T cell populations during a primary and a secondary CD8 T cell response. MHC-peptide tetramers were used to track the in vivo kinetics of expansion of T cell populations specific for two K-d-restricted epitopes simultaneously presented by a murine tumor cell following primary or recall immunizations. Individual syngeneic mice generated remarkably different primary CTL responses, as reflected by up to 60-fold differences in the relative contribution of each peptide-specific T cell population to the overall response. In these primary immunizations, the CTL dominance was not dictated by the respective abundance of the presented epitopes, In sharp contrast, the secondary response was systematically associated with a selective expansion of the same epitope-specific population both in vitro and in vivo. In vitro experiments indicated that the extent of expansion of each epitope-specific memory population is modulated by the epitope density. We conclude that, at least for this set of epitopes, the CTL hierarchy is not controlled by the same parameters in a primary vs a secondary response.
引用
收藏
页码:760 / 767
页数:8
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