A monoclonal blocking EIA for herpes simplex virus type 2 antibody: validation for seroepidemiological studies in Africa

被引:35
作者
Gopal, R
Gibbs, T
Slomka, MJ
Whitworth, J
Carpenter, LM
Vyse, A
Brown, DWG
机构
[1] Cent Publ Hlth Lab, Enter & Resp Virus Lab Virus, London NW9 5HT, England
[2] Uganda Virus Res Inst, MRC, Res Programme AIDS Uganda, Entebbe, Uganda
[3] Murex Biotech Ltd, Dartford, Kent, England
关键词
HSV-2; antibody; Africa; ELISA;
D O I
10.1016/S0166-0934(00)00149-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A competitive type-specific enzyme-linked immunosorbent assay (ELISA) for herpes simplex virus type 2 (HSV-2) antibody was developed using an infected cell antigen and a monoclonal antibody to glycoprotein G-2. This assay has been validated for use for epidemiological studies using a large panel of sera collected in rural Uganda and a panel of 143 sera characterised previously by Western blotting, the 'gold standard' for HSV type-specific serology. This evaluation was found to have a sensitivity of 96% and a specificity of 91% in comparison with Western blot on 143 sera from clinic patients. The ELISA had a sensitivity of 93% and a specificity of 91% in comparison with Western blot on 495 sera collected in Uganda. The assay showed good reproducibility and a low percentage of sera gave equivocal results, indicating its suitability for epidemiological studies. (C) 2000 Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:71 / 80
页数:10
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