Improved detection of microbial DNA after bead-beating before DNA isolation

被引:78
作者
de Boer, Richard [2 ]
Peters, Remco [1 ,3 ]
Gierveld, Sonja [1 ]
Schuurman, Tim [2 ]
Kooistra-Smid, Mirjam [2 ]
Savelkoul, Paul [1 ]
机构
[1] Vrije Univ Amsterdam Med Ctr, Dept Med Microbiol & Infect Control, NL-1007 MB Amsterdam, Netherlands
[2] Lab Infect Dis Groningen, Dept Res & Dev, Groningen, Netherlands
[3] Vrije Univ Amsterdam Med Ctr, Dept Internal Med, NL-1007 MB Amsterdam, Netherlands
关键词
Bead-beating; DNA isolation; MagNA Lyser; MagNA Pure; Qiagen column; Real-time PCR; REAL-TIME PCR; EXTRACTION; PURIFICATION; BACTERIA; SAMPLES; RNA;
D O I
10.1016/j.mimet.2009.11.009
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms. We demonstrated that physical disruption by bead-beating with 0.1 mm beads in combination with MagNA Pure DNA III extraction enhances microbial lysis of diverse Gram-positive microorganisms and may be used to optimize DNA extraction protocols in routine clinical diagnostics. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:209 / 211
页数:3
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