Improved detection of microbial DNA after bead-beating before DNA isolation

被引：78

作者：

de Boer, Richard ^{[2
]}

Peters, Remco ^{[1
,3
]}

Gierveld, Sonja ^{[1
]}

Schuurman, Tim ^{[2
]}

Kooistra-Smid, Mirjam ^{[2
]}

Savelkoul, Paul ^{[1
]}

机构：

[1] Vrije Univ Amsterdam Med Ctr, Dept Med Microbiol & Infect Control, NL-1007 MB Amsterdam, Netherlands

[2] Lab Infect Dis Groningen, Dept Res & Dev, Groningen, Netherlands

[3] Vrije Univ Amsterdam Med Ctr, Dept Internal Med, NL-1007 MB Amsterdam, Netherlands

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2010年 / 80卷 / 02期

关键词：

Bead-beating; DNA isolation; MagNA Lyser; MagNA Pure; Qiagen column; Real-time PCR; REAL-TIME PCR; EXTRACTION; PURIFICATION; BACTERIA; SAMPLES; RNA;

D O I：

10.1016/j.mimet.2009.11.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms. We demonstrated that physical disruption by bead-beating with 0.1 mm beads in combination with MagNA Pure DNA III extraction enhances microbial lysis of diverse Gram-positive microorganisms and may be used to optimize DNA extraction protocols in routine clinical diagnostics. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：209 / 211

页数：3

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