ARC POMC mRNA and PVN α-MSH are lower in obese relative to lean Zucker rats

被引:67
作者
Kim, EM
O'Hare, E
Grace, MK
Welch, CC
Billington, CJ
Levine, AS
机构
[1] VA Med Ctr, Res Serv 151, Minnesota Obes Ctr, Minneapolis, MN 55417 USA
[2] VA Med Ctr, Res Serv, Minneapolis, MN 55417 USA
[3] VA Med Ctr, Ctr Geriatr Res Educ & Clin, Minneapolis, MN 55417 USA
[4] Univ Minnesota, Dept Med, Minneapolis, MN 55455 USA
[5] Univ Minnesota, Dept Psychiat, Minneapolis, MN 55455 USA
[6] Univ Ulster, Dept Psychol, Jordanstown BT37 0QB, North Ireland
关键词
opioids; NPY; melanocortin; energy regulation;
D O I
10.1016/S0006-8993(00)02060-6
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Effects of obesity on gene expression for opioid peptides and neuropeptide-Y (NPY) in the arcuate nucleus (ARC), and on opioid peptides and alpha-melanocyte stimulating hormone (alpha-MSH) in the paraventricular nucleus (PVN) were examined in obese Zucker rats (18 weeks old). Obese Zucker rats are insulin-resistant, diabetic and hyperleptinemic as indicated by high serum glucose, insulin and leptin levels. ARC proOpiomelanocortin (POMC) mRNA levels were significantly lower in the obese relative to lean Zucker rats and ARC proNeuropeptide Y (proNPY) mRNA levels were higher (P<0.05). There were no differences in proDynorphin and proEnkephalin mRNA levels in the ARC (P > 0.05). Obese Zucker rats had lower alpha-MSH and dynorphin A(1-17) peptide levels in the paraventricular nucleus (PVN) (P < 0.05), but did not have lower PVN beta-endorphin peptide levels (P > 0.05). The decrease in POMC in the ARC and decrease in alpha-MSH in the PVN seen in the obese Zucker rat in the present study suggest that reduced activity of the melanocortin system in the ARC to PVN pathway may contribute to the related hyperphagia. Reduced activity of the melanocortin system in the ARC to PVN pathway may be due to a disturbance of leptin signaling coupling to POMC. (C) 2000 Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:11 / 16
页数:6
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