Hemoglobin niobate composite based biosensor for efficient determination of hydrogen peroxide in a broad pH range

被引：41

作者：

Gao, Lu ^{[1
]}

Gao, Qiuming ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Grad Sch, Shanghai 200050, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 07期

基金：

中国国家自然科学基金;

关键词：

biosensors; hemoglobin; layered niobates; direct electron transfer; hydrogen peroxide;

D O I：

10.1016/j.bios.2006.06.022

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Inorganic layered niobates (HCa2Nb3O10) were used as immobilization matrices of hemoglobin (Hb) because of their tunable interlayer spaces, large surface areas and good biocompatibilities. A pair of well-defined, quasi-reversible cycle voltammertric peaks were obtained at the Hb-HCa2Nb3O10 modified pyrolytic graphite electrode, suggesting that the layered niobates facilitate the electron transfer between the proteins and the electrode. Hb-HCa2Nb3O10 modified electrode exhibited electrocatalytic response for monitoring H2O2 with a large linear detection range from 25 mu M to 3.0 mM and a relatively high sensitivity of 172 mu A mM(-1) cm(-2). Based on the stabilizing effect of the layered niobates, Hb-HCa2Nb3O10 modified electrode can detect H2O2 in strongly acidic and basic solutions with pH of 1-12, which greatly expands the application fields of biosensors. (c) 2006 Elsevier B.V All rights reserved.

引用

页码：1454 / 1460

页数：7

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