Microfluidic digital PCR enables multigene analysis of individual environmental bacteria

被引：570

作者：

Ottesen, Elizabeth A.

Hong, Jong Wook

Quake, Stephen R.

Leadbetter, Jared R. ^{[1
]}

机构：

[1] CALTECH, Environm Sci & Engn Program, Pasadena, CA 91125 USA

[2] CALTECH, Div Biol, Pasadena, CA 91125 USA

[3] Auburn Univ, Samuel Ginn Coll Engn, Mat Res & Educ Ctr, Auburn, AL 36849 USA

[4] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[5] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA

来源：

SCIENCE | 2006年 / 314卷 / 5804期

关键词：

D O I：

10.1126/science.1131370

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gene inventory and metagenomic techniques have allowed rapid exploration of bacterial diversity and the potential physiologies present within microbial communities. However, it remains nontrivial to discover the identities of environmental bacteria carrying two or more genes of interest. We have used microfluidic digital polymerase chain reaction (PCR) to amplify and analyze multiple, different genes obtained from single bacterial cells harvested from nature. A gene encoding a key enzyme involved in the mutualistic symbiosis occurring between termites and their gut microbiota was used as an experimental hook to discover the previously unknown ribosomal RNA - based species identity of several symbionts. The ability to systematically identify bacteria carrying a particular gene and to link any two or more genes of interest to single species residing in complex ecosystems opens up new opportunities for research on the environment.

引用

页码：1464 / 1467

页数：4

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