Identification of a novel PP2C-type mitochondrial phosphatase

被引：29

作者：

Joshi, Mandar

Jeoung, Nam Ho

Popov, Kirill M.

Harris, Robert A. ^{[1
]}

机构：

[1] Indiana Univ, Sch Med, Dept Biochem & Mol Biol, Indianapolis, IN 46202 USA

[2] Univ Alabama Birmingham, Dept Biochem & Mol Genet, Birmingham, AL 35294 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 356卷 / 01期

关键词：

protein phosphatase 2C; mitochondria; branched-chain alpha-keto acid dehydrogenase;

D O I：

10.1016/j.bbrc.2007.02.108

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel phosphatase has been cloned and partially characterized. It has a mitochondrial leader sequence and its amino acid sequence places it in the PP2C family like two known mitochondrial phosphatases. Western blot analysis of subcellular fractions and confocal microscopy of 3T3L1 preadipocytes expressing the GFP-tagged protein confirm its mitochondrial localization. Western blot analysis indicates that the protein is expressed in several mouse tissues, with highest expression in brain, heart, liver, and kidney. The recombinant protein exhibits Mn2+-dependent phosphoserine phosphatase activity against the branched-chain alpha-keto acid dehydrogenase complex, suggesting the enzyme may play a role in regulation of branched chain amino acid catabolism. Whether there are other mitochondrial substrates for the enzyme is not known. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：38 / 44

页数：7

共 24 条

[1] Structural relationship between a bacterial developmental protein and eukaryotic PP2C protein phosphatases [J].