N-(beta-carboxypropionyl) luminol as a new chemiluminescence label in immunoassay

被引：5

作者：

Zhuang, HS ^{[1
]}

Wang, QE ^{[1
]}

Zhang, F ^{[1
]}

Huang, QJ ^{[1
]}

Tang, YC ^{[1
]}

机构：

[1] FUZHOU GEN HOSP,NANJING MIL REG,FUZHOU 350002,FUJIAN,PEOPLES R CHINA

来源：

CHINESE JOURNAL OF CHEMISTRY | 1997年 / 15卷 / 02期

关键词：

chemiluminescence immunoassay; N-(beta-carboxypropionyl)luminol; sheep anti-human IgG;

D O I：

10.1002/cjoc.19970150206

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new chemiluminescence label N-(beta-carboxypropionyl)luminol (CPL) was used to label sheep anti-human IgG (SaHIgG). The labeled antibody was stable and could be detected at least down to 10-(17)similar to 10(-16) mol. The molar incorporation ratio was estimated to be 0.26 mol of CPL per mol of SaHIgG. There were no apparent changes in the immunoreactivity of the labeled SaHIgG and in the quantum efficiency of the CPL after labeling.

引用

页码：123 / 129

页数：7

共 7 条

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