Translating metabolic exchange with imaging mass spectrometry

被引：192

作者：

Yang, Yu-Liang ^{[1
]}

Xu, Yuquan ^{[1
]}

Straight, Paul ^{[2
]}

Dorrestein, Pieter C. ^{[1
,3
,4
,5
]}

机构：

[1] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA

[2] Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA

[3] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA

[4] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

[5] Univ Calif San Diego, Ctr Marine Biotechnol & Biomed, La Jolla, CA 92093 USA

来源：

NATURE CHEMICAL BIOLOGY | 2009年 / 5卷 / 12期

关键词：

STREPTOMYCES-COELICOLOR; BACILLUS-SUBTILIS; NONRIBOSOMAL PEPTIDES; PRODUCT; IDENTIFICATION; MEGACOMPLEX;

D O I：

10.1038/nchembio.252

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Metabolic exchange between an organism and the environment, including interactions with neighboring organisms, is important for processes of organismal development. Here we develop and use thin-layer agar natural product MALDI-TOF imaging mass spectrometry of intact bacterial colonies grown on top of the MALDI target plate to study an interaction between two species of bacteria and provide direct evidence that Bacillus subtilis silences the defensive arsenal of Streptomyces coelicolor.

引用

页码：885 / 887

页数：3

共 25 条

[1] SUBTILOSIN-A, A NEW ANTIBIOTIC PEPTIDE PRODUCED BY BACILLUS-SUBTILIS-168 - ISOLATION, STRUCTURAL-ANALYSIS, AND BIOGENESIS [J].

BABASAKI, K ;

TAKAO, T ;

SHIMONISHI, Y ;

KURAHASHI, K .

JOURNAL OF BIOCHEMISTRY, 1985, 98 (03) :585-603

[2] Fruiting body formation by Bacillus subtilis [J].

Branda, SS ;

González-Pastor, JE ;

Ben-Yehuda, S ;

Losick, R ;

Kolter, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (20) :11621-11626

[3] The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis [J].

Butcher, Rebecca A. ;

Schroeder, Frank C. ;

Fischbach, Michael A. ;

Straightt, Paul D. ;

Kolter, Roberto ;

Walsh, Christopher T. ;

Clardy, Jon .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (05) :1506-1509

[4] Unusual Odd-Electron Fragments from Even-Electron Protonated Prodiginine Precursors Using Positive-Ion Electrospray Tandem Mass Spectrometry [J].

Chen, Kan ;

Rannulu, Nalaka S. ;

Cai, Yang ;

Lane, Pat ;

Liebl, Andrea L. ;

Rees, Bernard B. ;

Corre, Christophe ;

Challis, Gregory L. ;

Cole, Richard B. .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2008, 19 (12) :1856-1866

[5] MALDI imaging mass spectrometry: molecular snapshots of biochemical systems [J].

Cornett, Dale S. ;

Reyzer, Michelle L. ;

Chaurand, Pierre ;

Caprioli, Richard M. .

NATURE METHODS, 2007, 4 (10) :828-833

[6] 2-Alkyl-4-hydroxymethylfuran-3-carboxylic acids, antibiotic production inducers discovered by Streptomyces coelicolor genome mining [J].

Corre, Christophe ;

Song, Lijiang ;

O'Rourke, Sean ;

Chater, Keith F. ;

Challis, Gregory L. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (45) :17510-17515

[7] Visualizing the spatial distribution of secondary metabolites produced by marine cyanobacteria and sponges via MALDI-TOF imaging [J].

Esquenazi, Eduardo ;

Coates, Cameron ;

Simmons, Luke ;

Gonzalez, David ;

Gerwick, William H. ;

Dorrestein, Pieter C. .

MOLECULAR BIOSYSTEMS, 2008, 4 (06) :562-570

[8] Strategies to unravel the function of orphan biosynthesis pathways: recent examples and future prospects [J].

Gross, Harald .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 75 (02) :267-277

[9] Chemo- and regioselective peptide cyclization triggered by the N-terminal fatty acid chain length:: The recombinant cyclase of the calcium-dependent antibiotic from Streptomyces coelicolor [J].

Grünewald, J ;

Sieber, SA ;

Marahiel, MA .

BIOCHEMISTRY, 2004, 43 (10) :2915-2925

[10] Use of synthetic analogues in confirmation of structure of the peptide antibiotics Maltacines [J].

Hagelin, Gunnar ;

Indrevoll, Bard ;

Hoeg-Jensen, Thomas .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2007, 268 (2-3) :254-264

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