Hepatitis C virus negative strand RNA is not detected in peripheral blood mononuclear cells and viral sequences are identical to those in serum: a case against extrahepatic replication

被引：75

作者：

Laskus, T

Radkowski, M

Wang, LF

Cianciara, J

Vargas, H

Rakela, J

机构：

[1] UNIV PITTSBURGH,MED CTR,DIV GASTROENTEROL & HEPATOL,PITTSBURGH,PA 15213

[2] MUNICIPAL HOSP INFECT DIS,PL-01201 WARSAW,POLAND

[3] INST INFECT DIS,PL-01201 WARSAW,POLAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1997年 / 78卷

关键词：

D O I：

10.1099/0022-1317-78-11-2747

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Peripheral blood mononuclear cells (PBMCs) from 27 hepatitis C virus (HCV)-infected patients were analysed for the presence of HCV negative strand RNA with strand-specific Tth-based RT-PCR. No negative strand RNA was detected in any sample, and positive strand HCV sequences amplified from PBMCs were identical to those found in serum. These findings suggest that HCV does not replicate in PBMCs, and the presence of HCV sequences at this site is compatible with passive virus adsorption and/or contamination by circulating virus.

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页码：2747 / 2750

页数：4

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