Insertion polymorphism of transposable elements and population structure of Anopheles gambiae M and S molecular forms in Cameroon

被引：16

作者：

Boulesteix, M.

Simard, F.

Antonio-Nkondjio, C.

Awono-Ambene, H. P.

Fontenille, D.

Biemont, C. ^{[1
]}

机构：

[1] Univ Lyon 1, CNRS, Lab Biometrie & Biol Evolut, UMR 5558, F-69622 Villeurbanne, France

[2] OCEAC, Lab Rech Paludisme, Yaounde, Cameroon

[3] IRD, Unite Rech 016, F-34394 Montpellier 5, France

[4] IMPM, Inst Rech Med & Plantes Med, Ctr Rech Med, Yaounde, Cameroon

来源：

MOLECULAR ECOLOGY | 2007年 / 16卷 / 02期

关键词：

Anopheles gambiae; insertion polymorphism; molecular forms; population genetics; speciation; transposable elements;

D O I：

10.1111/j.1365-294X.2006.03150.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The insertion polymorphism of five transposable element (TE) families was studied by Southern blots in several populations of the M and S molecular forms of the mosquito Anopheles gambiae sensu stricto from southern Cameroon. We showed that the mean TE insertion site number and the within-population insertion site polymorphism globally differed between the M and S molecular forms. The comparison of the TE insertion profiles of the populations revealed a significant differentiation between these two molecular forms (0.163 < Phi(ST) < 0.371). We cloned several insertions of a non-LTR retrotransposon (Aara8) that were fixed in one form and absent in the other one. The only insertion that could be clearly located on a chromosome arm mapped to cytological division 6 of chromosome X, confirming the importance of this region in the ongoing speciation between the M and S molecular forms.

引用

页码：441 / 452

页数：12

共 59 条

[1] Absence of the kdr mutation in the molecular 'M' form suggests different pyrethroid resistance mechanisms in the malaria vector mosquito Anopheles gambiae s.s. [J].