A modular chitin-binding protease associated with hemocytes and hemolymph in the mosquito Anopheles gambiae

被引:60
作者
Danielli, A
Loukeris, TG
Lagueux, M
Müller, HM
Richman, A
Kafatos, FC
机构
[1] European Mol Biol Lab, D-69117 Heidelberg, Germany
[2] CNRS, Inst Biol Mol & Cellulaire, F-67084 Strasbourg, France
关键词
D O I
10.1073/pnas.97.13.7136
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Sp22D, a modular serine protease encompassing chitin binding, low density lipoprotein receptor, and scavenger receptor cysteine-rich domains, was identified by molecular cloning in the malaria vector, Anopheles gambiae. It is expressed in multiple body parts and during much of development, most intensely in hemocytes. The protein appears to be posttranslationally modified. Its integral. putatively glycosylated form is secreted in the hemolymph, whereas a smaller form potentially generated by proteolytic processing is associated with the tissues. Bacterial challenge or wounding result in low-level RNA induction, but the protein does not bind to bacteria, nor is its processing affected by infection. However, Sp22D binds to chitin with high affinity and undergoes transient changes in processing during pupal to adult metamorphosis; it may respond to exposure to naked chitin during tissue remodeling or damage.
引用
收藏
页码:7136 / 7141
页数:6
相关论文
共 23 条