Introduction of anaerobic dechlorinating bacteria into soil slurry microcosms and nested-PCR monitoring

被引:47
作者
ElFantroussi, S
Mahillon, J
Naveau, H
Agathos, SN
机构
[1] UNIV CATHOLIQUE LOUVAIN, UNITE GEN BIOL, B-1348 LOUVAIN, BELGIUM
[2] UNIV CATHOLIQUE LOUVAIN, LAB GENET MICROBIENNE, B-1348 LOUVAIN, BELGIUM
关键词
D O I
10.1128/AEM.63.2.806-811.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Desulfomonile tiedjei and Desulfitobacterium dehalogenans were chosen as model bacteria to demonstrate the introduction of an anaerobic microbia reductive dechlorination activity into nonsterile soil slurry microcosms by inoculation. De novo 3-chlorobenzoate dechlorination activity was established with the bacterium D. tiedjei in microcosms normally devoid of this dechlorination capacity. The addition of D. tiedjei to microcosms supplemented with 20 mM pyruvate as the cosubstrate resulted in total biotransformation of 1.5 mM 3-chlorobenzoate within 7 days. The introduction of the bacterium Desulfitobacterium dehalogenans into nonsterile microcosms resulted in a shortening of the period required for dechlorination activity to be established. In microcosms inoculated with Desulfitobacterium dehalogenans, total degradation of 6 mM 3-chloro-4-hydroxy-phenoxyacetic acid (3-Cl-4-OHPA) was observed after 4 days in contrast to the result in noninoculated microcosms, where the total degradation of 3-Cl-4-OHPA by indigenous microorganisms was observed after 11 days. Both externally introduced bacterial strains were detected in soil slurry microcosms by a nested-PCR methodology.
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页码:806 / 811
页数:6
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