Ultrastructural characterization of endoplasmic reticulum - Golgi transport containers (EGTC)

被引:47
作者
Horstmann, H
Ng, CP
Tang, BL
Hong, WJ
机构
[1] Inst Mol & Cell Biol, Membrane Biol Lab, Singapore 117609, Singapore
[2] Inst Mol & Cell Biol, NCA Lab, Singapore 117609, Singapore
关键词
COPI; COPII; EGTC; golgi; VSVG;
D O I
10.1242/jcs.00115
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Recent observations made in live cells expressing green fluorescent protein (GFP)-tagged cargo markers have demonstrated the existence of large, mobile transport intermediates linking peripheral ER exit sites (ERES) to the perinuclear Golgi. Using a procedure of rapid ethane freezing, we examined ultrastructurally the intermediates involved in ER-Golgi transport of the vesicular stomatitis virus (VSV) G protein. When released at the permissive temperature of 32degreesC, VSVG is first found to be concentrated in pleiomorphic, membrane-bound structures (of about 0.4 to 1 mum in diameter) with extensive budding profiles. These structures are devoid of COPII components and Golgi markers, but are enriched in COPI, the retrograde cargo ERGIC53, and the tethering protein p115. The structures appear to be able to undergo fusion with the Golgi stack and are tentatively referred to as ER-Golgi transport containers, or EGTCs. VSVG. protein exiting the ERES at 15degreesC is first found in clusters or strings of COPII-containing small vesicles, and morphological analysis indicates that these clusters and strings of COPH vesicles may coalesce by homotypic fusion to form the EGTCs. Together with the large transport containers mediating transport from the trans-Golgi network to the plasma membrane, EGTCs represents an emerging class of large membranous structures mediating anterograde transport between the major stations of the exocytic pathway.
引用
收藏
页码:4263 / 4273
页数:11
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