Promoters P3, Pa/Pb, P4, and P5 upstream from blaTEM genes and their relationship to β-lactam resistance

被引：66

作者：

Lartigue, MF

Leflon-Guibout, W

Poirel, L

Nordmann, P

Nicolas-Chanoine, MH

机构：

[1] Hop Ambroise Pare, Assitance Publ Hop Paris, Serv Microbiol Hyg, F-92100 Boulogne, France

[2] Univ Versailles, Fac Paris Ile France Ouest, St Quentin en Yvelines, France

[3] Hop Bicetre, Assistance Publ Hop Paris, Serv Bacteriol Virol, Le Kremlin Bicetre, France

[4] Univ Paris 11, Fac Med, Paris, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2002年 / 46卷 / 12期

关键词：

D O I：

10.1128/AAC.46.12.4035-4037.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Using an isogenic system, we have determined the impact that the four promoters known to control bla(TEM) gene expression have on P-lactamase activity. For both TEM-1 and TEM-30, this activity gradually increased in relation to the presence of promoters P3, Pa/Pb, and P4 upstream of the corresponding gene. Promoter P5, only found upstream of the bla(TEM-1B) gene, was related to the highest expression of this gene.

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页码：4035 / 4037

页数：3

相关论文

共 10 条

[1] Inhibitor-resistant TEM β-lactamases:: phenotypic, genetic and biochemical characteristics [J].

Chaïbi, EB ;

Sirot, D ;

Paul, G ;

Labia, R .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1999, 43 (04) :447-458

[2] 2 IMPROVED PROMOTER SEQUENCES FOR THE BETA-LACTAMASE EXPRESSION ARISING FROM A SINGLE BASE-PAIR SUBSTITUTION [J].

CHEN, ST ;

CLOWES, RC .

NUCLEIC ACIDS RESEARCH, 1984, 12 (07) :3219-3234

[3] Updated sequence information for TEM β-lactamase genes [J].

Goussard, S ;

Courvalin, P .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (02) :367-370

[4] PROMOTER SELECTIVITY OF ESCHERICHIA-COLI RNA-POLYMERASE .8. PROMOTER SELECTIVITY OF ESCHERICHIA-COLI RNA-POLYMERASE - EFFECT OF BASE SUBSTITUTIONS IN THE PROMOTER -35 REGION ON PROMOTER STRENGTH [J].

KOBAYASHI, M ;

NAGATA, K ;

ISHIHAMA, A .

NUCLEIC ACIDS RESEARCH, 1990, 18 (24) :7367-7372

[5] Biochemical-genetic analysis and distribution of FAR-1, a class A β-lactamase from Nocardia farcinica [J].

Laurent, F ;

Poirel, L ;

Naas, T ;

Chaibi, E ;

Labia, R ;

Boiron, P ;

Nordmann, P .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (07) :1644-1650

[6] Epidemiological survey of amoxicillin-clavulanate resistance and corresponding molecular mechanisms in Escherichia coli isolates in France:: New genetic features of blaTEM genes [J].

Leflon-Guibout, V ;

Speldooren, V ;

Heym, B ;

Nicolas-Chanoine, MH .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (10) :2709-2714

[7] Updated sequence information and proposed nomenclature for blaTEM genes and their promoters [J].

Leflon-Guibout, V ;

Heym, B ;

Nicolas-Chanoine, MH .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (11) :3232-3234

[8] MCRA AND MCRB RESTRICTION PHENOTYPES OF SOME ESCHERICHIA-COLI STRAINS AND IMPLICATIONS FOR GENE CLONING [J].

RALEIGH, EA ;

MURRAY, NE ;

REVEL, H ;

BLUMENTHAL, RM ;

WESTAWAY, D ;

REITH, AD ;

RIGBY, PWJ ;

ELHAI, J ;

HANAHAN, D .

NUCLEIC ACIDS RESEARCH, 1988, 16 (04) :1563-1575

[9]

Soussy CJ, 2000, PATHOL BIOL, V48, P832

[10] NUCLEOTIDE-SEQUENCE OF AMPICILLIN RESISTANCE GENE OF ESCHERICHIA-COLI PLASMID PBR322 [J].

SUTCLIFFE, JG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (08) :3737-3741