Investigating membrane protein dynamics in living cells

被引：26

作者：

Bates, Ian R.

Wiseman, Paul W.

Hanrahan, John W.

机构：

[1] McGill Univ, Dept Physiol, Montreal, PQ H3G 1Y6, Canada

[2] McGill Univ, Dept Chem, Montreal, PQ H3A 2K6, Canada

来源：

BIOCHEMISTRY AND CELL BIOLOGY | 2006年 / 84卷 / 06期

关键词：

cystic fibrosis; image correlation spectroscopy; FRAP; single-particle tracking; fluorescence-correlation spectroscopy; chloride channel; epithelium; membrane domains;

D O I：

10.1139/O06-189

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of mernbrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.

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收藏

页码：825 / 831

页数：7

相关论文

共 23 条

[1] Fluorescence cross-correlation spectroscopy in living cells [J].

Bacia, K ;

Kim, SA ;

Schwille, P .

NATURE METHODS, 2006, 3 (02) :83-89

[2] A dynamic view of cellular processes by in vivo fluorescence auto- and cross-correlation spectroscopy [J].

Bacia, K ;

Schwille, P .

METHODS, 2003, 29 (01) :74-85

[3] Probing the endocytic pathway in live cells using dual-color fluorescence cross-correlation analysis [J].

Bacia, K ;

Majoul, IV ;

Schwille, P .

BIOPHYSICAL JOURNAL, 2002, 83 (02) :1184-1193

[4] Quantitative analysis of the formation and diffusion of A1-adenosine receptor-antagonist complexes in single living cells [J].

Briddon, SJ ;

Middleton, RJ ;

Cordeaux, Y ;

Flavin, FM ;

Weinstein, JA ;

George, MW ;

Kellam, B ;

Hill, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (13) :4673-4678

[5] Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking [J].

Dahan, M ;

Lévi, S ;

Luccardini, C ;

Rostaing, P ;

Riveau, B ;

Triller, A .

SCIENCE, 2003, 302 (5644) :442-445

[6] Increased diffusional mobility of CFTR at the plasma membrane after deletion of its C-terminal PDZ binding motif [J].

Haggie, PM ;

Stanton, BA ;

Verkman, AS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (07) :5494-5500

[7] Spatiotemporal image correlation Spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells [J].

Hebert, B ;

Costantino, S ;

Wiseman, PW .

BIOPHYSICAL JOURNAL, 2005, 88 (05) :3601-3614

[8] Paradigm shift of the plasma membrane concept from the two-dimensional continuum fluid to the partitioned fluid: High-speed single-molecule tracking of membrane molecules [J].

Kusumi, A ;

Nakada, C ;

Ritchie, K ;

Murase, K ;

Suzuki, K ;

Murakoshi, H ;

Kasai, RS ;

Kondo, J ;

Fujiwara, T .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 2005, 34 :351-U54

[9] THE DYNAMIC STRUCTURE OF THE PERICELLULAR MATRIX ON LIVING CELLS [J].

LEE, GM ;

JOHNSTONE, B ;

JACOBSON, K ;

CATERSON, B .

JOURNAL OF CELL BIOLOGY, 1993, 123 (06) :1899-1907

[10] Regulation of protein mobility in cell membranes: A dynamic corral model [J].

Leitner, DM ;

Brown, FLH ;

Wilson, KR .

BIOPHYSICAL JOURNAL, 2000, 78 (01) :125-135